Association between circulating mononuclear cell mitochondrial DNA copy number and in-hospital mortality in septic patients: A prospective observational study based on the Sepsis-3 definition

To explore the association between circulating mononuclear cell mitochondrial DNA copy number and the prognosis of sepsis patients based on the Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3 definition). A total of 200 adult patients who had recently devoloped sepsis...

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Published in:PloS one 2019-02, Vol.14 (2), p.e0212808-e0212808
Main Authors: Yang, Yi, Yang, Jingjuan, Yu, Biying, Li, Li, Luo, Lin, Wu, Fengfeng, Wu, Binbin
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Analysis
Biology and life sciences
Cell survival
Cell-Free Nucleic Acids - blood
Chinese medicine
Copy number
Demographics
Deoxyribonucleic acid
Disease prevention
DNA
DNA, Mitochondrial - blood
EDTA
Genetic research
Genomes
Hospital Mortality
Humans
Infection
Leukocytes, Mononuclear
Medical prognosis
Medical research
Medicine and Health Sciences
Middle Aged
Mitochondrial DNA
Mortality
Observational studies
Patient outcomes
Patients
Polymerase chain reaction
Prognosis
Prospective Studies
Research and analysis methods
Sepsis
Sepsis - blood
Sepsis - mortality
Septic shock
Shock
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creator Yang, Yi
Yang, Jingjuan
Yu, Biying
Li, Li
Luo, Lin
Wu, Fengfeng
Wu, Binbin
description To explore the association between circulating mononuclear cell mitochondrial DNA copy number and the prognosis of sepsis patients based on the Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3 definition). A total of 200 adult patients who had recently devoloped sepsis were prospectively recruited as the study cohort. Demographic and clinical data were recorded along with a 28-day outcome. Mononuclear cell mtDNA copy number was assessed by quantitative PCR. The 28-day outcome of sepsis patients was significantly associated with circulating mononuclear cell mtDNA copy number. The median mononuclear cell relative mtDNA copy number of survivors was significantly higher than that of nonsurvivors (406.68, range 196.65-625.35 vs. 320.57, range 175.98-437.33, p = 0.001). The Cox proportional hazard survival model analysis indicated that mononuclear cell relative mtDNA copy number was significantly negative associated with the 28-day outcome. For every additional unit of mononuclear cell mtDNA relative copy number, the risk of death falls by 0.1% (HR = 0.999, 95% CI = 0.998 to 1.000, p = 0.017). Our data indicate first that circulating mononuclear cellular mtDNA copy number might be helpful for outcome predictions in sepsis patients, and second that lower mtDNA copy number implied poor prognosis.
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A total of 200 adult patients who had recently devoloped sepsis were prospectively recruited as the study cohort. Demographic and clinical data were recorded along with a 28-day outcome. Mononuclear cell mtDNA copy number was assessed by quantitative PCR. The 28-day outcome of sepsis patients was significantly associated with circulating mononuclear cell mtDNA copy number. The median mononuclear cell relative mtDNA copy number of survivors was significantly higher than that of nonsurvivors (406.68, range 196.65-625.35 vs. 320.57, range 175.98-437.33, p = 0.001). The Cox proportional hazard survival model analysis indicated that mononuclear cell relative mtDNA copy number was significantly negative associated with the 28-day outcome. For every additional unit of mononuclear cell mtDNA relative copy number, the risk of death falls by 0.1% (HR = 0.999, 95% CI = 0.998 to 1.000, p = 0.017). Our data indicate first that circulating mononuclear cellular mtDNA copy number might be helpful for outcome predictions in sepsis patients, and second that lower mtDNA copy number implied poor prognosis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30794688</pmid><doi>10.1371/journal.pone.0212808</doi><tpages>e0212808</tpages><orcidid>https://orcid.org/0000-0002-6285-0807</orcidid><oa>free_for_read</oa></addata></record>
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subjects Adult
Aged
Aged, 80 and over
Analysis
Biology and life sciences
Cell survival
Cell-Free Nucleic Acids - blood
Chinese medicine
Copy number
Demographics
Deoxyribonucleic acid
Disease prevention
DNA
DNA, Mitochondrial - blood
EDTA
Genetic research
Genomes
Hospital Mortality
Humans
Infection
Leukocytes, Mononuclear
Medical prognosis
Medical research
Medicine and Health Sciences
Middle Aged
Mitochondrial DNA
Mortality
Observational studies
Patient outcomes
Patients
Polymerase chain reaction
Prognosis
Prospective Studies
Research and analysis methods
Sepsis
Sepsis - blood
Sepsis - mortality
Septic shock
Shock
title Association between circulating mononuclear cell mitochondrial DNA copy number and in-hospital mortality in septic patients: A prospective observational study based on the Sepsis-3 definition
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