Fluid dynamics during bleb formation in migrating cells in vivo

Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We sho...

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Bibliographic Details
Published in:PloS one 2019-02, Vol.14 (2), p.e0212699-e0212699
Main Authors: Goudarzi, Mohammad, Boquet-Pujadas, Aleix, Olivo-Marin, Jean-Christophe, Raz, Erez
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified - embryology
Animals, Genetically Modified - genetics
Apoptosis
Apoptosis - physiology
Aquaporins
Biology
Biology and Life Sciences
Cancer metastasis
Cell adhesion & migration
Cell migration
Cell Movement - physiology
Cell size
Computational fluid dynamics
Cytokinesis
Cytokinesis - physiology
Cytoplasm
Cytoplasm - genetics
Cytoplasm - metabolism
Embryos
Fluid dynamics
Germ cells
Germ Cells - cytology
Germ Cells - metabolism
Homeostasis
Hydrodynamics
Inflow
Models, Biological
Physical Sciences
Physiological aspects
Regulation
Research and Analysis Methods
Zebrafish
Zebrafish - embryology
Zebrafish - genetics
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Summary:Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0212699