Aqueous extract of Hibiscus sabdariffa inhibits pedestal induction by enteropathogenic E. coli and promotes bacterial filamentation in vitro

Diarrheic diseases account for the annual death of approximately 1.9 million children under the age of 5 years, and it is a major cause of work absenteeism in developed countries. As diarrheagenic bacteria, enteropathogenic Escherichia coli (EPEC) attach to cells in the small intestine, causing loca...

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Published in:PloS one 2019-03, Vol.14 (3), p.e0213580-e0213580
Main Authors: Mohamed-Salem, Reda, Rodríguez Fernández, Carmina, Nieto-Pelegrín, Elvira, Conde-Valentín, Beatriz, Rumbero, Angel, Martinez-Quiles, Narcisa
Format: Article
Language:English
Subjects:
Absenteeism
Absenteeism (Labor)
Acids
Actin
Analysis
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Anti-infective agents
Antibacterial activity
Antibacterial agents
Antibiotics
Antimicrobial agents
Bacteria
Bactericidal activity
Bioactive compounds
Biology and Life Sciences
Care and treatment
Children
Death
Developed countries
Diarrhea
E coli
Enterotoxigenic Escherichia coli - growth & development
Enterotoxigenic Escherichia coli - ultrastructure
Escherichia coli
Filamentation
Filaments
Food
Hibiscus
Hibiscus - chemistry
Hibiscus sabdariffa
Industrialized countries
Kinetics
Medicine and Health Sciences
Microorganisms
Minimum inhibitory concentration
Muscle proteins
Parasitology
Pathogens
Pharmacy
Phosphorylation
Plant Extracts - chemistry
Plant Extracts - pharmacology
Polymerization
Prevention
Properties
Proteins
Research and analysis methods
Sepsis
Small intestine
Sulbactam
Tazobactam
Tetracyclines
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cited_by cdi_FETCH-LOGICAL-c692t-36e2c178a00ad4922bec745dd2a87c8aca25344b994212e7f95160ba02611583
cites cdi_FETCH-LOGICAL-c692t-36e2c178a00ad4922bec745dd2a87c8aca25344b994212e7f95160ba02611583
container_end_page e0213580
container_issue 3
container_start_page e0213580
container_title PloS one
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creator Mohamed-Salem, Reda
Rodríguez Fernández, Carmina
Nieto-Pelegrín, Elvira
Conde-Valentín, Beatriz
Rumbero, Angel
Martinez-Quiles, Narcisa
description Diarrheic diseases account for the annual death of approximately 1.9 million children under the age of 5 years, and it is a major cause of work absenteeism in developed countries. As diarrheagenic bacteria, enteropathogenic Escherichia coli (EPEC) attach to cells in the small intestine, causing local disappearance of microvilli and inducing the formation of actin-rich pedestals that disrupt the intestinal barrier and help EPEC adhere to and infect intestinal cells. Antibiotics and other bioactive compounds can often be found by analyzing traditional medicines. Here a crude aqueous extract of Hibiscus sabdariffa, which typically grows in subtropical and tropical areas and is a popular medicinal tisane in many countries, was analyzed for antibacterial activity against EPEC. In standard microdilution assays, the extract showed a minimum inhibitory concentration of 6.5 mg/ml against EPEC growth. Time-kill kinetics assays demonstrated significant 24 h bactericidal activity at 25 mg/ml. The extract is able to impede pedestal induction. Not only did the extract inhibit preformed pedestals but it prevented pedestal induction as well. Remarkably, it also promoted the formation of EPEC filaments, as observed with other antibiotics. Our results in vitro support the potential of Hibiscus sabdariffa as an antimicrobial agent against EPEC.
doi_str_mv 10.1371/journal.pone.0213580
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As diarrheagenic bacteria, enteropathogenic Escherichia coli (EPEC) attach to cells in the small intestine, causing local disappearance of microvilli and inducing the formation of actin-rich pedestals that disrupt the intestinal barrier and help EPEC adhere to and infect intestinal cells. Antibiotics and other bioactive compounds can often be found by analyzing traditional medicines. Here a crude aqueous extract of Hibiscus sabdariffa, which typically grows in subtropical and tropical areas and is a popular medicinal tisane in many countries, was analyzed for antibacterial activity against EPEC. In standard microdilution assays, the extract showed a minimum inhibitory concentration of 6.5 mg/ml against EPEC growth. Time-kill kinetics assays demonstrated significant 24 h bactericidal activity at 25 mg/ml. The extract is able to impede pedestal induction. Not only did the extract inhibit preformed pedestals but it prevented pedestal induction as well. Remarkably, it also promoted the formation of EPEC filaments, as observed with other antibiotics. Our results in vitro support the potential of Hibiscus sabdariffa as an antimicrobial agent against EPEC.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30849110</pmid><doi>10.1371/journal.pone.0213580</doi><tpages>e0213580</tpages><orcidid>https://orcid.org/0000-0002-4396-4016</orcidid><orcidid>https://orcid.org/0000-0002-0366-6591</orcidid><oa>free_for_read</oa></addata></record>
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subjects Absenteeism
Absenteeism (Labor)
Acids
Actin
Analysis
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Anti-infective agents
Antibacterial activity
Antibacterial agents
Antibiotics
Antimicrobial agents
Bacteria
Bactericidal activity
Bioactive compounds
Biology and Life Sciences
Care and treatment
Children
Death
Developed countries
Diarrhea
E coli
Enterotoxigenic Escherichia coli - growth & development
Enterotoxigenic Escherichia coli - ultrastructure
Escherichia coli
Filamentation
Filaments
Food
Hibiscus
Hibiscus - chemistry
Hibiscus sabdariffa
Industrialized countries
Kinetics
Medicine and Health Sciences
Microorganisms
Minimum inhibitory concentration
Muscle proteins
Parasitology
Pathogens
Pharmacy
Phosphorylation
Plant Extracts - chemistry
Plant Extracts - pharmacology
Polymerization
Prevention
Properties
Proteins
Research and analysis methods
Sepsis
Small intestine
Sulbactam
Tazobactam
Tetracyclines
title Aqueous extract of Hibiscus sabdariffa inhibits pedestal induction by enteropathogenic E. coli and promotes bacterial filamentation in vitro
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