Effects of drought-stress on seed germination and growth physiology of quinclorac-resistant Echinochloa crusgalli

• Published in: PloS one 2019-04, Vol.14 (4), p.e0214480-e0214480
• Main Authors: Wu, La-Mei, Fang, Yong, Yang, Hao-Na, Bai, Lian-Yang
• Format: Article
• Language: English
• Subjects: Analysis; Biology and Life Sciences; Biotypes; China; Crop yield; Drought; Droughts; Echinochloa; Echinochloa - drug effects; Echinochloa - enzymology; Ecology and Environmental Sciences; Gene Expression Regulation, Plant - drug effects; Germination; Germination - drug effects; Glutathione; Glutathione transferase; Glutathione Transferase - metabolism; Glycols (Class of compounds); Grasses; Herbicide Resistance; Herbicides; Herbicides - pharmacology; Influence; Lyases - metabolism; Oryza - metabolism; Physiological aspects; Plant Proteins - metabolism; Plant Roots - metabolism; Plant Weeds - drug effects; Plant Weeds - enzymology; Polyethylene glycol; Polyethylene Glycols; Polyols; Quinolines - pharmacology; Research and Analysis Methods; Rice; Roots; Seed germination; Seeds; Seeds - drug effects; Shoots; Spectrophotometry; Stress (Physiology); Stress, Physiological; Transferases; Weeds; Weight
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0214480

Echinochloa crusgalli (L.) Beauv. (barnyard grass) is considered a noxious weed worldwide, and is the most pernicious weed decreasing rice yields in China. Recently, E. crusgalli has evolved quinclorac resistance, making it among the most serious herbicide resistant weeds in China. The present study explored differences in germination and growth between quinclorac-resistant and -susceptible E. crusgalli collected in Hunan Province. The order of the seven E. crusgalli biotypes assessed, from high to low quinclorac-resistance, was: quinclorac-resistant, Chunhua, Hanshou, Shimen, Hekou, Dingcheng, and quinclorac-susceptible. With an increased in the level of quinclorac-resistance, the germination rate, length of young shoots and roots, and fresh weight of E. crusgalli were all decreased compared with that in more susceptible biotypes. However, there were no significant differences between quinclorac-resistant and susceptible E. crusgalli biotypes without polyethylene glycol 6000 treatment. Drought had a more obvious effect on glutathione S-transferases (GST) activity, determined by spectrophotometric method, in quinclorac-resistant E. crusgalli. Higher resistance level biotypes showed greater activity, and when treated with polyethylene glycol 6000 for 3 days, all E. crusgalli biotypes showed the highest GST activity. This study demonstrated that as the level of quinclorac-resistance increased, the rate of seed germination decreased, while the growth of young buds, young roots, and fresh weight decreased. Increased quinclorac-resistance may be related to the increased metabolic activity of GST in E. crusgalli.