Genetic diversity, and description of a new dagger nematode, Xiphinema afratakhtehnsis sp. nov., (Dorylaimida: Longidoridae) in natural forests of southeastern Gorgan, northern Iran

• Published in: PloS one 2019-05, Vol.14 (5), p.e0214147-e0214147
• Main Authors: Fouladvand, Zeinab Mirzaie, Pourjam, Ebrahim, Castillo, Pablo, Pedram, Majid
• Format: Article
• Language: English
• Subjects: Animals; Bayesian analysis; Biodiversity; Biology and Life Sciences; Computer and Information Sciences; Cytochrome; Cytochrome c; Cytochrome oxidase; Cytochrome-c oxidase; Cytochromes; Deoxyribonucleic acid; Developmental stages; DNA; Ecology and Environmental Sciences; EDTA; Female; Females; Forests; Genes; Genetic aspects; Genetic diversity; Genetic research; Genetic Variation - genetics; Haplotypes; Iran; Juveniles; Males; Medicine and Health Sciences; Mitochondria; Mitochondrial DNA; Morphology; Nematoda - classification; Nematoda - genetics; Nematodes; New species; Oxidases; People and Places; Phylogenetics; Phylogeny; Physical characteristics; Physiological aspects; Plant pathology; Ribosomal RNA; RNA; Roundworms; rRNA; Spacer; Spicules; Spines; Statistical inference; Trees; Vulva; Xiphinema
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0214147

The most prevalent dagger nematode recovered from rhizospheric soil samples of forest trees in the Afrātakhteh region of Golestan province (Iran) was Xiphinema afratakhtehnsis sp. nov. and it is described and illustrated with integrative approaches using both morphological and molecular criteria. It belongs to the morphospecies group 6 of the intragenic historical grouping of Xiphinema non-americanum species. The new species is characterized by females with 3.3-4.9 mm sized body, lip region separated from the rest of body by a depression, anteriorly expanded, 16-18 μm wide, vulva located at 47.2-58.5%, odontostyle 155-173 μm and odontophore 89-107 μm long, female genital system composed of two equally developed branches, the tubular part of each having spines, short symmetrically rounded female tail to symmetrically rounded with a small mucro-like projection at the end in a few females, rare males (n = 1 out of 74 females) with 83 μm long dorylaimoid spicules and four juvenile developmental stages. The third-stage juveniles (J3) have a characteristic tail shape (short, symmetrically conical with a club-shaped long mucro) demarcating the species, and being typologically useful for its separation from closely similar species (except X. cohni, with currently no data on its juvenile stages) viz. X. adenohystherum, X. iranicum, X. mazandaranense, X. nuragicum, X. pyrenaicum, X. robbinsi, X. sphaerocephalum and X. zagrosense. Molecular phylogenetic studies using genomic (partial large subunit and internal transcribed spacer 1 ribosomal RNA genes: D2-D3 and ITS1 rDNA) and mitochondrial cytochrome c oxidase subunit I gene (COI mtDNA) revealed the new species forming a unique lineage in all reconstructed trees using Bayesian inference (BI) and maximum likelihood (ML) methods. The sequenced isolates of the new species formed a monophyletic group in the D2-D3 tree. The sequenced isolates of the new species for their COI mtDNA formed four subclades in COI mtDNA phylogeny, and four haplotypes in corresponding analysis.