Assessment of TCF7L2 expression after bariatric surgery

• Published in: PloS one 2019-05, Vol.14 (5), p.e0216627-e0216627
• Main Authors: Macedo, Carlos Eduardo S, da Conti, Guilherme, Catena, Andriu S, Bruneska, Danyelly, Rosa, Malu, Noronha, Clarissa G, Santa Cruz, Fernando, Ferraz, Álvaro A B
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Analysis; Bariatric surgery; Bariatric Surgery - methods; Biology and Life Sciences; Body mass; Body Mass Index; Body size; Correlation; Deoxyribonucleic acid; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - etiology; Diabetes Mellitus, Type 2 - surgery; Diabetics; DNA; Female; Gastrointestinal surgery; Gene expression; Genetic engineering; Health aspects; Humans; Influence; Laboratories; Laparoscopy; Male; Medicine and Health Sciences; Metabolism; Middle Aged; Obesity; Obesity - blood; Obesity - complications; Obesity - surgery; Patient outcomes; Peripheral blood; Physical Sciences; Postoperative Period; Prospective Studies; Regression analysis; Regression models; Research and Analysis Methods; Subgroups; Surgery; Transcription Factor 7-Like 2 Protein - blood; Transcription Factor 7-Like 2 Protein - genetics; Transcription factors; Treatment Outcome; Type 2 diabetes; Weight Loss; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0216627

To assess the influence of bariatric surgery on transcription factor 7-like 2 (TCF7L2) expression and its association with body mass index (BMI) and Type 2 diabetes mellitus (T2DM). Prospective study performed between 2016 and 2018, where 26 obese patients undergoing bariatric surgery were divided into two subgroups: diabetics and non-diabetics. The RNAs were extracted from peripheral blood samples that were obtained from each patient in two different moments: before surgery and after 12 months of follow-up. The relative expression of TCF7L2 was determined according to the delta-Ct method. The linear regression model of BMI x delta-Ct showed a positive correlation (p = 0.037). In the subgroups, an inversely proportional relationship was found between delta-Ct and BMI in the diabetic group and a directly proportional relationship in the non-diabetic group (p>0.05 in both). In the postoperative period, the regression model was similar to the preoperative, except when analyzing the subgroups, where diabetic patients showed a directly proportional relationship (p>0.05). The relative expression of TCF7L2 showed an average of 1.16 ± 0.91, CI-95% 0.79-1.53. There was an increase in relative expression of 48% in the non-diabetic group (p = 0.021), and a decrease of 27% in the T2DM group (p>0.05) in the postoperative. There was a positive correlation between a greater decrease in BMI and increased relative expression (p = 0.027). Our results showed that generally, the TCF7L2 expression increase with a decrease in BMI, however, for patients with T2DM, it exhibits an inverse pattern, which is normalized one year after bariatric surgery.