MicroRNA-582-5p suppresses non-small cell lung cancer cells growth and invasion via downregulating NOTCH1

• Published in: PloS one 2019-06, Vol.14 (6), p.e0217652-e0217652
• Main Authors: Liu, Jianghong, Liu, Shengshuo, Deng, Xiaoyan, Rao, Jiaoyu, Huang, Kaiyuan, Xu, Gengrui, Wang, Xiaokang
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions - genetics; Analysis; Base Sequence; Biological activity; Biology and Life Sciences; Biomarkers; Cancer cells; Carcinoma; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Care and treatment; Cell cycle; Cell growth; Cell Line, Tumor; Cell proliferation; Cell Proliferation - genetics; Cellular signal transduction; Down-Regulation - genetics; Ectopic expression; Female; Gene expression; Gene Expression Regulation, Neoplastic; Gene Silencing; Genetic aspects; Growth factors; Growth rate; Humans; Invasiveness; Kinases; Lung cancer; Lung diseases; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Male; Medical prognosis; Medical research; Medicine and Health Sciences; MicroRNA; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; miRNA; Neoplasm Invasiveness; Non-small cell lung cancer; Non-small cell lung carcinoma; Notch1 protein; Pharmacy; Receptor, Notch1 - genetics; Receptor, Notch1 - metabolism; Research and Analysis Methods; Restoration; Ribonucleic acid; RNA; Signal transduction; Small cell lung cancer; Tissues; Tumors; Up-Regulation - genetics
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0217652

Non-small cell lung cancer (NSCLC) is the most common cancer worldwide. MicroRNAs have been shown to be correlated with biological processes of various tumors. In this study, we observed that the expression of miR-582-5p was lower in NSCLC tissues than that in para-carcinoma tissues. Ectopic expression of miR-582-5p significantly inhibited NCI-H358 cell proliferation and invasion. Knockdown of miR-582-5p showed the opposite results, with cell growth rate and the invasive capacity of PC-9 cells enhanced. Furthermore, we elucidated that NOTCH1 is a target of miR-582-5p and there is an inverse correlation between miR-582-5p and NOTCH1 expression in NSCLC tissues. Overexpression of NOTCH1 in miR-582-5p-overexpressing NCI-H358 cells could partially reverse the inhibition of cell proliferation and invasion by miR-582-5p. Thus, our research demonstrated that miR-582-5p suppresses NSCLC cell lines' growth and invasion via targeting oncoprotein NOTCH1 and restoration of miR-582-5p might be feasible therapeutic strategy for NSCLC.