The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera

This study presents an evaluation of four tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, GRA1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric prote...

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Published in:PloS one 2019-06, Vol.14 (6), p.e0217866-e0217866
Main Authors: Ferra, Bartłomiej Tomasz, Holec-Gąsior, Lucyna, Gatkowska, Justyna, Dziadek, Bożena, Dzitko, Katarzyna, Grąźlewska, Weronika, Lautenbach, Dariusz
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Protozoan - immunology
Antigens
Antigens, Protozoan - immunology
Assaying
Avidity
Biology and Life Sciences
Care and treatment
Chronic infection
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzymes
Fragments
Genetic engineering
Genetically modified organisms
Humans
Immunoglobulin G
Immunoglobulin G - immunology
Immunoglobulin M
Immunoglobulin M - immunology
Immunoglobulins
Infection
Infections
Laboratories
Medicine and Health Sciences
Mice
Proteins
Protozoa
Protozoan Proteins - immunology
Recombinant Fusion Proteins - immunology
Research and Analysis Methods
Risk factors
Rodents
Sensitivity and Specificity
Serologic Tests - methods
Toxoplasma
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis
Toxoplasmosis - immunology
Womens health
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cited_by cdi_FETCH-LOGICAL-c758t-697999ca38b74f7db4a26cca5522893e61331f4321b7e6589d05768463a365463
cites cdi_FETCH-LOGICAL-c758t-697999ca38b74f7db4a26cca5522893e61331f4321b7e6589d05768463a365463
container_end_page e0217866
container_issue 6
container_start_page e0217866
container_title PloS one
container_volume 14
creator Ferra, Bartłomiej Tomasz
Holec-Gąsior, Lucyna
Gatkowska, Justyna
Dziadek, Bożena
Dzitko, Katarzyna
Grąźlewska, Weronika
Lautenbach, Dariusz
description This study presents an evaluation of four tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, GRA1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG2-GRA1-ROP1-AMA1N, AMA1N-SAG2-GRA1-ROP1, AMA1C-SAG2-GRA1-ROP1, and AMA1-SAG2-GRA1-ROP1) obtained by genetic engineering were tested for their reactivity with specific IgM and IgG antibodies from sera of experimentally infected mice and humans with T. gondii infection using an enzyme-linked immunosorbent assay (ELISA). In total 192 serum samples from patients with acquired T. gondii infection and 137 sera from seronegative individuals were examined. The reactivity of chimeric antigens with antibodies generated during T. gondii invasion was measured and compared to the results obtained in assays based on whole-cell Toxoplasma antigen. Chimeric proteins proved effective in differentiation between T. gondii-infected and uninfected individuals (100% sensitivity and specificity in the IgG ELISAs) which shows their potential usefulness as a replacements for TLA in standardized commercial tests for the serodiagnosis of toxoplasmosis. In addition, the chimeric proteins were tested for use in avidity determination. Obtained results were comparable to those of the corresponding commercial assays, suggesting the utility of these proteins for avidity assessment. Furthermore, this study demonstrated that the AMA1-SAG2-GRA1-ROP1 chimeric protein has the potential to distinguish specific antibodies from serum samples of individuals with the early and chronic phase of T. gondii infection.
doi_str_mv 10.1371/journal.pone.0217866
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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferra, Bartłomiej Tomasz</au><au>Holec-Gąsior, Lucyna</au><au>Gatkowska, Justyna</au><au>Dziadek, Bożena</au><au>Dzitko, Katarzyna</au><au>Grąźlewska, Weronika</au><au>Lautenbach, Dariusz</au><au>Ho, Paulo Lee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-06-06</date><risdate>2019</risdate><volume>14</volume><issue>6</issue><spage>e0217866</spage><epage>e0217866</epage><pages>e0217866-e0217866</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study presents an evaluation of four tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, GRA1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG2-GRA1-ROP1-AMA1N, AMA1N-SAG2-GRA1-ROP1, AMA1C-SAG2-GRA1-ROP1, and AMA1-SAG2-GRA1-ROP1) obtained by genetic engineering were tested for their reactivity with specific IgM and IgG antibodies from sera of experimentally infected mice and humans with T. gondii infection using an enzyme-linked immunosorbent assay (ELISA). In total 192 serum samples from patients with acquired T. gondii infection and 137 sera from seronegative individuals were examined. The reactivity of chimeric antigens with antibodies generated during T. gondii invasion was measured and compared to the results obtained in assays based on whole-cell Toxoplasma antigen. Chimeric proteins proved effective in differentiation between T. gondii-infected and uninfected individuals (100% sensitivity and specificity in the IgG ELISAs) which shows their potential usefulness as a replacements for TLA in standardized commercial tests for the serodiagnosis of toxoplasmosis. In addition, the chimeric proteins were tested for use in avidity determination. Obtained results were comparable to those of the corresponding commercial assays, suggesting the utility of these proteins for avidity assessment. Furthermore, this study demonstrated that the AMA1-SAG2-GRA1-ROP1 chimeric protein has the potential to distinguish specific antibodies from serum samples of individuals with the early and chronic phase of T. gondii infection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31170254</pmid><doi>10.1371/journal.pone.0217866</doi><tpages>e0217866</tpages><orcidid>https://orcid.org/0000-0001-6420-4721</orcidid><orcidid>https://orcid.org/0000-0002-0559-1706</orcidid><orcidid>https://orcid.org/0000-0003-2176-3966</orcidid><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
ispartof PloS one, 2019-06, Vol.14 (6), p.e0217866-e0217866
issn 1932-6203
1932-6203
language eng
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source Publicly Available Content Database; PubMed Central
subjects Animals
Antibodies
Antibodies, Protozoan - immunology
Antigens
Antigens, Protozoan - immunology
Assaying
Avidity
Biology and Life Sciences
Care and treatment
Chronic infection
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzymes
Fragments
Genetic engineering
Genetically modified organisms
Humans
Immunoglobulin G
Immunoglobulin G - immunology
Immunoglobulin M
Immunoglobulin M - immunology
Immunoglobulins
Infection
Infections
Laboratories
Medicine and Health Sciences
Mice
Proteins
Protozoa
Protozoan Proteins - immunology
Recombinant Fusion Proteins - immunology
Research and Analysis Methods
Risk factors
Rodents
Sensitivity and Specificity
Serologic Tests - methods
Toxoplasma
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis
Toxoplasmosis - immunology
Womens health
title The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera
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