ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation

• Published in: PloS one 2019-06, Vol.14 (6), p.e0217079
• Main Authors: Arshad, Rabia, Sohail, Muhammad Farhan, Sarwar, Hafiz Shoaib, Saeed, Hamid, Ali, Imran, Akhtar, Sohail, Hussain, Syed Zajif, Afzal, Iqra, Jahan, Sarwat, Anees-Ur-Rehman, Shahnaz, Gul
• Format: Article
• Language: English
• Subjects: Alginates; Alginic acid; Analysis; Animal models; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibacterial agents; Antibiotics; Bandages; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Biocompatible Materials - pharmacology; Biocompatible Materials - therapeutic use; Biodegradability; Biodegradation; Biology and Life Sciences; Biomedical materials; Care and treatment; Cell adhesion & migration; Cell Survival - drug effects; Chemical compounds; Chitosan; College campuses; Complications; Dressings; Engineering and Technology; HeLa Cells; Humans; Hydrogels; Infection; Infections; Infectious diseases; Lysozyme; Medical dressings; Medicine and Health Sciences; Muramidase - metabolism; Nanoparticles; Nanoparticles - chemistry; Patient compliance; Pharmaceutical sciences; Pharmacology; Pharmacy; Physical Sciences; Porosity; Postoperative complications; Research and Analysis Methods; Risk factors; Safety regulations; Skin - drug effects; Skin - pathology; Staphylococcus aureus - drug effects; Sulfhydryl Compounds - chemistry; Surgery; Surgical dressings; Surgical site infections; Surgical Wound Infection - drug therapy; Surgical Wound Infection - pathology; Surgical Wound Infection - physiopathology; Transplants & implants; Wound care; Wound healing; Wound Healing - drug effects; Wounds; Zinc; Zinc oxide; Zinc Oxide - chemistry; Zinc oxides; Zinc sulfate
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0217079

Post-operative surgical site infections (SSI) present a serious threat and may lead to complications. Currently available dressings for SSI lack mucoadhesion, safety, efficacy and most importantly patient compliance. We aimed to address these concerns by developing a bioactive thiolated chitosan-alginate bandage embedded with zinc oxide nanoparticles (ZnO-NPs) for localized topical treatment of SSI. The FTIR, XRD, DSC and TGA of bandage confirmed the compatibility of ingredients and modifications made. The porosity, swelling index and lysozyme degradation showed good properties for wound healing and biodegradation. Moreover, in-vitro antibacterial activity showed higher bactericidal effect as compared to ZnO-NPs free bandage. In-vivo wound healing in murine model showed significant improved tissue generation and speedy wound healing as compared to positive and negative controls. Over all, thiolated bandage showed potential as an advanced therapeutic agent for treating surgical site infections, meeting the required features of an ideal surgical dressing.