Hyaluronan-based hydrogels as dermal fillers: The biophysical properties that translate into a "volumetric" effect

Biophysical and biochemical data on hyaluronan (HA)-based dermal fillers strongly support their optimal use and design to meet specific requisites. Here, four commercially available (in Europe) HA "volumetric" fillers, among the most used in the clinical practice, have been characterized i...

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Published in:PloS one 2019-06, Vol.14 (6), p.e0218287-e0218287
Main Authors: La Gatta, Annalisa, Salzillo, Rosanna, Catalano, Claudia, D'Agostino, Antonella, Pirozzi, Anna Virginia Adriana, De Rosa, Mario, Schiraldi, Chiara
Format: Article
Language:English
Subjects:
Acids
Aquaporins
Backup software
Biochemistry
Biological effects
Biology and Life Sciences
Biopolymers
Cell Line
Collagen
Collagen (type I)
Collagen Type I - metabolism
Degradation
Dermal fillers
Dermal Fillers - chemistry
Dermal Fillers - pharmacology
Elasticity
Elastin
Extracellular matrix
Fibroblasts
Fibroblasts - cytology
Fibroblasts - metabolism
Fillers
Gels
Humans
Hyaluronic acid
Hyaluronic Acid - chemistry
Hyaluronic Acid - pharmacology
Hydrates
Hydrogels
Hydrogels - chemistry
Hydrogels - pharmacology
Materials Testing
Medicine
Optimization
Oxygen
Physical Sciences
Properties
Reactive oxygen species
Rheological properties
Rigidity
Skin
Skin care products
Viscosity
Volumetric analysis
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cited_by cdi_FETCH-LOGICAL-c692t-3c232fc7f58e1d45c0d1441f0cca7619cd83575dfbe9eb9eca577bf105f678e13
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container_issue 6
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container_title PloS one
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creator La Gatta, Annalisa
Salzillo, Rosanna
Catalano, Claudia
D'Agostino, Antonella
Pirozzi, Anna Virginia Adriana
De Rosa, Mario
Schiraldi, Chiara
description Biophysical and biochemical data on hyaluronan (HA)-based dermal fillers strongly support their optimal use and design to meet specific requisites. Here, four commercially available (in Europe) HA "volumetric" fillers, among the most used in the clinical practice, have been characterized in vitro. Analyses revealed the highest amounts of water-soluble HA reported so far and provided hydrodynamic data for these soluble polymeric fractions. Volumetric gels exhibit a wide range of rigidity with most of them showing G' values around 200-300Pa. They greatly differ in cohesivity. 1mL of gel hydrates up to 2.4-3.2mL. The products completely solubilize due to Bovine Testicular Hyaluronidase (BTH)'s action, thus predicting in vivo complete resorption. For the first time, filler degradation due to reactive oxygen species (ROS) was studied by rheological measurements and a rank in stability was established. Studies using Human Dermal Fibroblasts (HDF) indicated a positive biological response to the HA networks. Further, gel capacity to prompt collagen I, elastin and aquaporin3 synthesis was demonstrated, thus suggesting a positive effect on skin elasticity and hydration, besides the physical volumetric action. The findings are the first wide assessment of features for the volumetric class of HA-fillers and include first data on their resistance to degradation by ROS and biological effects on HDF. The study represents a valuable contribution to the understanding of HA-fillers, useful to optimize their use and manufacture.
doi_str_mv 10.1371/journal.pone.0218287
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subjects Acids
Aquaporins
Backup software
Biochemistry
Biological effects
Biology and Life Sciences
Biopolymers
Cell Line
Collagen
Collagen (type I)
Collagen Type I - metabolism
Degradation
Dermal fillers
Dermal Fillers - chemistry
Dermal Fillers - pharmacology
Elasticity
Elastin
Extracellular matrix
Fibroblasts
Fibroblasts - cytology
Fibroblasts - metabolism
Fillers
Gels
Humans
Hyaluronic acid
Hyaluronic Acid - chemistry
Hyaluronic Acid - pharmacology
Hydrates
Hydrogels
Hydrogels - chemistry
Hydrogels - pharmacology
Materials Testing
Medicine
Optimization
Oxygen
Physical Sciences
Properties
Reactive oxygen species
Rheological properties
Rigidity
Skin
Skin care products
Viscosity
Volumetric analysis
title Hyaluronan-based hydrogels as dermal fillers: The biophysical properties that translate into a "volumetric" effect
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