Podocin and uPAR are good biomarkers in cases of Focal and segmental glomerulosclerosis in pediatric renal biopsies

• Published in: PloS one 2019-06, Vol.14 (6), p.e0217569-e0217569
• Main Authors: Pereira, Lívia Helena de Morais, da Silva, Crislaine Aparecida, Monteiro, Maria Luíza Gonçalves Dos Reis, Araújo, Liliane Silvano, Rocha, Laura Penna, Reis, Marcelo Bernardes da Rocha, Ramalho, Fernando Silva, Corrêa, Rosana Rosa Miranda, Silva, Marcos Vinicius, Reis, Marlene Antonia, Machado, Juliana Reis
• Format: Article
• Language: English
• Subjects: Adolescent; Analysis; Autopsies; Autopsy; Bioindicators; Biological markers; Biology and Life Sciences; Biomarkers; Biomarkers - metabolism; Biopsy; Case-Control Studies; Cell adhesion & migration; Child; Child health; Child, Preschool; Diagnosis; Diagnosis, Differential; Diagnostic systems; Discipline; Disease; Female; Gene Expression; Glomerulosclerosis, Focal Segmental - diagnosis; Glomerulosclerosis, Focal Segmental - genetics; Glomerulosclerosis, Focal Segmental - metabolism; Glomerulosclerosis, Focal Segmental - pathology; Humans; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Kidney biopsy; Kidney diseases; Kidney Glomerulus - metabolism; Kidney Glomerulus - pathology; Lesions; Male; Medical research; Medicine and Health Sciences; Membrane proteins; Membrane Proteins - genetics; Membrane Proteins - metabolism; Methods; Nephrosis, Lipoid - diagnosis; Nephrosis, Lipoid - genetics; Nephrosis, Lipoid - metabolism; Nephrosis, Lipoid - pathology; Pathology; Pediatrics; Physical Sciences; Predictive Value of Tests; Proteins; Receptors, Urokinase Plasminogen Activator - genetics; Receptors, Urokinase Plasminogen Activator - metabolism; Renal function; Research and Analysis Methods; Sclerosis; Sensitivity; Transplants & implants; U-Plasminogen activator; Urokinase; WT1 Proteins - genetics; WT1 Proteins - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0217569

There are controversies whether Minimal Change Disease (MCD) and Focal and Segmental Glomerulosclerosis (FSGS) are distinct glomerular lesions or different manifestations within the same spectrum of diseases. The uPAR (urokinase-type plasminogen activator receptor) and some slit diaphragm proteins may be altered in FSGS glomeruli and may function as biomarkers of the disease in renal biopsies. Thus, this study aims to evaluate the diagnostic potential of uPAR and glomerular proteins for differentiation between MCD and FSGS in renal pediatric biopsy. Renal biopsies from 50 children between 2 and 18 years old were selected, with diagnosis of MCD (n = 29) and FSGS (n = 21). Control group consisted of pediatric autopsies (n = 15) from patients younger than 18 years old, with no evidences of renal dysfunction. In situ expressions of WT1, nephrin, podocin and uPAR were evaluated by immunoperoxidase technique. Renal biopsy of patients with MCD and FSGS expressed fewer WT1 (p≤0.0001, F = 19.35) and nephrin (p