Dietary Alaska pollack protein improves skeletal muscle weight recovery after immobilization-induced atrophy in rats

• Published in: PloS one 2019-06, Vol.14 (6), p.e0217917-e0217917
• Main Authors: Fujitani, Mina, Mizushige, Takafumi, Kawabata, Fuminori, Uozumi, Keisuke, Yasui, Machi, Hayamizu, Kohsuke, Uchida, Kenji, Okada, Shinji, Keshab, Bhattarai, Kishida, Taro
• Format: Article
• Language: English
• Subjects: Alaska; Alaska pollock; Anesthesia; Animals; Atrophy; Biology and Life Sciences; Body fat; Body weight gain; Casein; Casts, Surgical; Cell cycle; Comparative analysis; Composition; Diet; Diet therapy; Dietary protein; Extremities - physiopathology; Feeding; Fish Proteins, Dietary - pharmacology; Gene expression; Genes; Health; Health aspects; High fat diet; Immobilization; Immobilization - physiology; Insulin-like growth factor I; Insulin-Like Growth Factor I - metabolism; Insulin-like growth factors; Kinases; Laboratory animals; Limbs; Male; Medicine and Health Sciences; Milk proteins; Muscle Proteins - metabolism; Muscle recovery; Muscle, Skeletal - drug effects; Muscles; Muscular atrophy; Muscular Atrophy - drug therapy; Musculoskeletal system; Nutrition research; Plasters; Pollachius pollachius; Pollack; Protein synthesis; Proteins; Rats; Rats, Sprague-Dawley; Recovery; Recovery (Medical); Rodents; Skeletal muscle; Soleus muscle; Testing
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0217917

The promotion of muscle recovery after immobilization is important to preserve an optimum health status. Here, we examined the effect of dietary Alaska pollack protein (APP) on skeletal muscle weight after atrophy induced by hind limb immobilization using plaster immobilization technique. Rat left limb was casted with a wetted plaster cast under anesthesia. After 2 weeks of feeding, the cast was removed and the rats were divided into three groups, namely, a baseline group, high-fat casein diet group, and high-fat APP diet group. After 3 weeks of feeding, the skeletal muscles (soleus, extensor digitorum longus [EDL], and gastrocnemius) were sampled. The estimated weight gains of soleus, gastrocnemius, and EDL muscle in the immobilized limbs were significantly larger in the rats fed with APP diet as compared with those fed with casein diet. In soleus muscle, dietary APP increased the expression of Igf1 and Myog genes in the immobilized limbs after the recovery period.