Evolthon: A community endeavor to evolve lab evolution

• Published in: PLoS biology 2019-03, Vol.17 (3), p.e3000182
• Main Authors: Kaminski Strauss, Sivan, Schirman, Dvir, Jona, Ghil, Brooks, Aaron N, Kunjapur, Aditya M, Nguyen Ba, Alex N, Flint, Alice, Solt, Andras, Mershin, Andreas, Dixit, Atray, Yona, Avihu H, Csörgő, Bálint, Busby, Bede Phillip, Hennig, Bianca P, Pál, Csaba, Schraivogel, Daniel, Schultz, Daniel, Wernick, David G, Agashe, Deepa, Levi, Dikla, Zabezhinsky, Dmitry, Russ, Dor, Sass, Ehud, Tamar, Einat, Herz, Elad, Levy, Emmanuel D, Church, George M, Yelin, Idan, Nachman, Iftach, Gerst, Jeffrey E, Georgeson, Joseph M, Adamala, Katarzyna P, Steinmetz, Lars M, Rübsam, Marc, Ralser, Markus, Klutstein, Michael, Desai, Michael M, Walunjkar, Nilima, Yin, Ning, Aharon Hefetz, Noa, Jakimo, Noah, Snitser, Olga, Adini, Omri, Kumar, Prashant, Soo Hoo Smith, Rachel, Zeidan, Razi, Hazan, Ronen, Rak, Roni, Kishony, Roy, Johnson, Shannon, Nouriel, Shira, Vonesch, Sibylle C, Foster, Simmie, Dagan, Tal, Wein, Tanita, Karydis, Thrasyvoulos, Wannier, Timothy M, Stiles, Timothy, Olin-Sandoval, Viridiana, Mueller, William F, Bar-On, Yinon M, Dahan, Orna, Pilpel, Yitzhak
• Format: Article
• Language: English
• Subjects: Adaptation; Baking yeast; Biochemistry; Biological Evolution; Biological research; Biology and Life Sciences; Biology, Experimental; Brewer's yeast; Communities; Dentistry; E coli; Environmental conditions; Environmental science; Escherichia coli; Escherichia coli - metabolism; Evolution; Evolution & development; Evolutionary biology; Exploration; Gene expression; Genetic diversity; Genomes; Humans; Laboratories; Life sciences; Low temperature; Medical schools; Medicine; Models, Genetic; Molecular biology; Mutagenesis; Mutation; Mutation - genetics; Mutation rates; Natural history; Organic chemistry; Physical Sciences; Physics; Research and Analysis Methods; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Scientists; Strains (organisms); Temperature
• ISSN: 1545-7885; 1544-9173; 1545-7885
• DOI: 10.1371/journal.pbio.3000182

In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best to evolve a desired trait? Should the challenge be applied abruptly, gradually, periodically, sporadically? Should one apply chemical mutagenesis, and do strains with high innate mutation rate evolve faster? What are ideal population sizes of evolving populations? There are endless strategies, beyond those that can be exposed by individual labs. We therefore arranged a community challenge, Evolthon, in which students and scientists from different labs were asked to evolve Escherichia coli or Saccharomyces cerevisiae for an abiotic stress-low temperature. About 30 participants from around the world explored diverse environmental and genetic regimes of evolution. After a period of evolution in each lab, all strains of each species were competed with one another. In yeast, the most successful strategies were those that used mating, underscoring the importance of sex in evolution. In bacteria, the fittest strain used a strategy based on exploration of different mutation rates. Different strategies displayed variable levels of performance and stability across additional challenges and conditions. This study therefore uncovers principles of effective experimental evolutionary regimens and might prove useful also for biotechnological developments of new strains and for understanding natural strategies in evolutionary arms races between species. Evolthon constitutes a model for community-based scientific exploration that encourages creativity and cooperation.