Narya, a RING finger domain-containing protein, is required for meiotic DNA double-strand break formation and crossover maturation in Drosophila melanogaster

• Published in: PLoS genetics 2019-01, Vol.15 (1), p.e1007886-e1007886
• Main Authors: Lake, Cathleen M, Nielsen, Rachel J, Bonner, Amanda M, Eche, Salam, White-Brown, Sanese, McKim, Kim S, Hawley, R Scott
• Format: Article
• Language: English
• Subjects: Animals; Beef cattle; Binding proteins; Biology and Life Sciences; Chromosomes; CRISPR; Crossing Over, Genetic; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded; DNA damage; Drosophila; Drosophila melanogaster - genetics; Evolution; Gene duplication; Genes; Genetic aspects; Genetics; Genomes; Homologous Recombination - genetics; Insects; Mammals; Medical research; Meiosis; Meiosis - genetics; Molecular biology; Nodules; Observations; Oocytes - growth & development; Oocytes - metabolism; Organisms; Phylogenetics; Physiological aspects; Protein Domains - genetics; Proteins; Recombination; Research and Analysis Methods; RING Finger Domains - genetics; RNA Interference; Two-Hybrid System Techniques; X Chromosome - genetics; Yeast
• ISSN: 1553-7404; 1553-7390; 1553-7404
• DOI: 10.1371/journal.pgen.1007886

Meiotic recombination, which is necessary to ensure that homologous chromosomes segregate properly, begins with the induction of meiotic DNA double-strand breaks (DSBs) and ends with the repair of a subset of those breaks into crossovers. Here we investigate the roles of two paralogous genes, CG12200 and CG31053, which we have named Narya and Nenya, respectively, due to their relationship with a structurally similar protein named Vilya. We find that narya recently evolved from nenya by a gene duplication event, and we show that these two RING finger domain-containing proteins are functionally redundant with respect to a critical role in DSB formation. Narya colocalizes with Vilya foci, which are known to define recombination nodules, or sites of crossover formation. A separation-of-function allele of narya retains the capacity for DSB formation but cannot mature those DSBs into crossovers. We further provide data on the physical interaction of Narya, Nenya and Vilya, as assayed by the yeast two-hybrid system. Together these data support the view that all three RING finger domain-containing proteins function in the formation of meiotic DNA DSBs and in the process of crossing over.