A systematic genetic screen identifies essential factors involved in nuclear size control

Nuclear size correlates with cell size, but the mechanism by which this scaling is achieved is not known. Here we screen fission yeast gene deletion mutants to identify essential factors involved in this process. Our screen has identified 25 essential factors that alter nuclear size, and our analysi...

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Bibliographic Details
Published in:PLoS genetics 2019-02, Vol.15 (2), p.e1007929
Main Authors: Cantwell, Helena, Nurse, Paul
Format: Article
Language:English
Subjects:
Biology and Life Sciences
Cell cycle
Cell division
Cell nucleus
Cell Nucleus - genetics
Cell Size
Clonal deletion
Control
Crick, Francis
Cytoplasm - genetics
Cytoskeleton - genetics
Deletion mutant
Deoxyribonucleic acid
DNA
Gene Deletion
Gene Expression Regulation, Fungal - genetics
Genes
Genetic screening
Genetic testing
Genetic Testing - methods
Genomes
Genotype & phenotype
Influence
Laboratories
Measurement
Methods
Nuclear DNA
Ontology
Phenotype
Phenotypes
Research and Analysis Methods
RNA processing
Scaling
Schizosaccharomyces - genetics
Switch genes
Yeast
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Summary:Nuclear size correlates with cell size, but the mechanism by which this scaling is achieved is not known. Here we screen fission yeast gene deletion mutants to identify essential factors involved in this process. Our screen has identified 25 essential factors that alter nuclear size, and our analysis has implicated RNA processing and LINC complexes in nuclear size control. This study has revealed lower and more extreme higher nuclear size phenotypes and has identified global cellular processes and specific structural nuclear components important for nuclear size control.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1007929