Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization

• Published in: PLoS pathogens 2019-03, Vol.15 (3), p.e1007381-e1007381
• Main Authors: Murdoch, Caitlin C, Espenschied, Scott T, Matty, Molly A, Mueller, Olaf, Tobin, David M, Rawls, John F
• Format: Article
• Language: English
• Subjects: Amyloid; Animal genetic engineering; Animals; Artificial intelligence; Bacteria; Bactericidal activity; Biology and Life Sciences; Biomarkers; Cell activation; Colonization; Danio rerio; Disease; Epithelial cells; Funding; Gene expression; Genetics; Genomes; Gnotobiotic; Homeostasis; Immune clearance; Immune system; Immunity; Immunity, Innate - physiology; In vivo methods and tests; Infections; Inflammation; Innate immunity; Intestinal microflora; Intestine; Intestines; Leukocytes (neutrophilic); Liver; Machine learning; Mammals; Medicine; Medicine and Health Sciences; Microbiota; Microbiota (Symbiotic organisms); Molecular modelling; Mutants; Neutrophil Activation - physiology; Neutrophilia; Neutrophils; Neutrophils - physiology; Phenotypes; Proteins; Research and Analysis Methods; Serum Amyloid A Protein - metabolism; Serum Amyloid A Protein - physiology; Signal Transduction; Tissues; Vertebrates; Wounding; Wounds; Zebrafish; Zebrafish - microbiology
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1007381

The intestinal microbiota influences the development and function of myeloid lineages such as neutrophils, but the underlying molecular mechanisms are unresolved. Using gnotobiotic zebrafish, we identified the immune effector Serum amyloid A (Saa) as one of the most highly induced transcripts in digestive tissues following microbiota colonization. Saa is a conserved secreted protein produced in the intestine and liver with described effects on neutrophils in vitro, however its in vivo functions remain poorly defined. We engineered saa mutant zebrafish to test requirements for Saa on innate immunity in vivo. Zebrafish mutant for saa displayed impaired neutrophil responses to wounding but augmented clearance of pathogenic bacteria. At baseline, saa mutants exhibited moderate neutrophilia and altered neutrophil tissue distribution. Molecular and functional analyses of isolated neutrophils revealed that Saa suppresses expression of pro-inflammatory markers and bactericidal activity. Saa's effects on neutrophils depended on microbiota colonization, suggesting this protein mediates the microbiota's effects on host innate immunity. To test tissue-specific roles of Saa on neutrophil function, we over-expressed saa in the intestine or liver and found that sufficient to partially complement neutrophil phenotypes observed in saa mutants. These results indicate Saa produced by the intestine in response to microbiota serves as a systemic signal to neutrophils to restrict aberrant activation, decreasing inflammatory tone and bacterial killing potential while simultaneously enhancing their ability to migrate to wounds.