Dissociation of natriuresis and diuresis by oxytocin molecular forms in rats

In the rat, oxytocin (OT) produces dose-dependent diuretic and natriuretic responses. Post-translational enzymatic conversion of the OT biosynthetic precursor forms both mature and C-terminally extended peptides. The plasma concentrations of these C-terminally extended peptides (OT-G; OT-GK and OT-G...

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Bibliographic Details
Published in:PloS one 2019-07, Vol.14 (7), p.e0219205-e0219205
Main Authors: Jankowski, Marek, Danalache, Bogdan A, Plante, Eric, Menaouar, Ahmed, Florian, Maria, Tan, Ju Jing, Grygorczyk, Ryszard, Broderick, Tom L, Gutkowska, Jolanta
Format: Article
Language:English
Subjects:
Animals
Antidiuretics
Arginine
Argipressin
Autoradiography
Binding
Binding sites
Binding, Competitive
Biology and Life Sciences
Cell Line
CHO Cells
Cricetinae
Cricetulus
Cyclic adenosine monophosphate
Cyclic AMP
Cyclic AMP - metabolism
Dissociation
Diuresis
Diuretics
EDTA
Electrolytes
Electrolytes - metabolism
Endocrinology
Enzymes
Excretion
Experiments
Heart
Homeostasis
Humans
Injection
Intravenous administration
Kidney - metabolism
Kidneys
Laboratories
Medicine
Medicine and Health Sciences
Methods
Molecular docking
Molecular Docking Simulation
Natriuresis
Neonates
Oxytocin
Oxytocin - metabolism
Peptides
Peptides - metabolism
Physical Sciences
Physiology
Post-translation
Potassium
Rats
Rats, Wistar
Reabsorption
Receiving waters
Receptors, Vasopressin - metabolism
Research and Analysis Methods
Sodium
Urination
Urine
Vasopressin
Vasopressin V2 receptors
Vasopressins - metabolism
Water
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Summary:In the rat, oxytocin (OT) produces dose-dependent diuretic and natriuretic responses. Post-translational enzymatic conversion of the OT biosynthetic precursor forms both mature and C-terminally extended peptides. The plasma concentrations of these C-terminally extended peptides (OT-G; OT-GK and OT-GKR) are elevated in newborns and pregnant rats. Intravenous injection of OT-GKR to rats inhibits diuresis, whereas injection of amidated OT stimulates diuresis. Since OT and OT-GKR show different effects on the urine flow, we investigated whether OT-GKR modulates renal action by inhibition of the arginine-vasopressin (AVP) receptor V2 (V2R), the receptor involved in renal water reabsorption. Experiments were carried out in the 8-week-old Wistar rats receiving intravenous (iv) injections of vehicle, OT, OT-GKR or OT+OT-GKR combination. OT (10 μmol/kg) increased urine outflow by 40% (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0219205