Reprogramming of Trypanosoma cruzi metabolism triggered by parasite interaction with the host cell extracellular matrix

• Published in: PLoS neglected tropical diseases 2019-02, Vol.13 (2), p.e0007103
• Main Authors: Mattos, Eliciane C, Canuto, Gisele, Manchola, Nubia C, Magalhães, Rubens D M, Crozier, Thomas W M, Lamont, Douglas J, Tavares, Marina F M, Colli, Walter, Ferguson, Michael A J, Alves, Maria Júlia M
• Format: Article
• Language: English
• Subjects: Amastigotes; Animals; Binding; Biology and Life Sciences; Cell cycle; Cell differentiation; Cells (Biology); Cellular signal transduction; Cellulose; Chagas disease; Cytoskeleton; Dehydrogenases; Deoxyribonucleic acid; DNA; Enzymes; Etiology; Extracellular; Extracellular matrix; Extracellular Matrix - parasitology; Flagella; Fructose; Gas Chromatography-Mass Spectrometry; Gene Expression Regulation - physiology; Glucose; Glycolysis; Heparan sulfate; Hexokinase; Host-Parasite Interactions; Humans; Incubation period; Infections; Infective stages; Kinases; L-Lactate dehydrogenase; Lactate dehydrogenase; Lactic acid; Life sciences; Mammalian cells; Mammals; Metabolism; Metabolites; Monosaccharides; Nucleic acids; Parasites; Phosphatase; Phosphofructokinase; Phosphoglucomutase; Phosphoglycerate kinase; Phosphoproteins - metabolism; Phosphorylation; Physical Sciences; Proteases; Protein binding; Proteins; Proteome - metabolism; Proteomes; Protozoa; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Pyruvate kinase; Pyruvic acid; Ribonucleic acid; RNA; RNA transport; Signal transduction; Switches; Tissue; Tropical diseases; Trypanosoma cruzi; Trypanosoma cruzi - metabolism; Trypomastigotes; Vector-borne diseases; Vertebrates
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0007103

Trypanosoma cruzi, the etiological agent of Chagas' disease, affects 8 million people predominantly living in socioeconomic underdeveloped areas. T. cruzi trypomastigotes (Ty), the classical infective stage, interact with the extracellular matrix (ECM), an obligatory step before invasion of almost all mammalian cells in different tissues. Here we have characterized the proteome and phosphoproteome of T. cruzi trypomastigotes upon interaction with ECM (MTy) and the data are available via ProteomeXchange with identifier PXD010970. Proteins involved with metabolic processes (such as the glycolytic pathway), kinases, flagellum and microtubule related proteins, transport-associated proteins and RNA/DNA binding elements are highly represented in the pool of proteins modified by phosphorylation. Further, important metabolic switches triggered by this interaction with ECM were indicated by decreases in the phosphorylation of hexokinase, phosphofructokinase, fructose-2,6-bisphosphatase, phosphoglucomutase, phosphoglycerate kinase in MTy. Concomitantly, a decrease in the pyruvate and lactate and an increase of glucose and succinate contents were detected by GC-MS. These observations led us to focus on the changes in the glycolytic pathway upon binding of the parasite to the ECM. Inhibition of hexokinase, pyruvate kinase and lactate dehydrogenase activities in MTy were observed and this correlated with the phosphorylation levels of the respective enzymes. Putative kinases involved in protein phosphorylation altered upon parasite incubation with ECM were suggested by in silico analysis. Taken together, our results show that in addition to cytoskeletal changes and protease activation, a reprogramming of the trypomastigote metabolism is triggered by the interaction of the parasite with the ECM prior to cell invasion and differentiation into amastigotes, the multiplicative intracellular stage of T. cruzi in the vertebrate host.