Relationship of transcriptional markers to Leydig cell number in the mouse testis

The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically established the...

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Bibliographic Details
Published in:PloS one 2019-07, Vol.14 (7), p.e0219524-e0219524
Main Authors: Rebourcet, Diane, Monteiro, Ana, Cruickshanks, Lyndsey, Jeffery, Nathan, Smith, Sarah, Milne, Laura, O'Shaughnessy, Peter J, Smith, Lee B
Format: Article
Language:English
Subjects:
Ablation
Androgens
Animals
Animals, Newborn
Biodiversity
Bioindicators
Biological markers
Biology and Life Sciences
Biomarkers
Biomarkers - metabolism
Cell Count - methods
Cell number
Cell size
Decision analysis
Decision making
Endocrinology
Gene Expression Profiling
Genes
Laboratory rats
Leydig Cells
Male
Medical research
Medicine and Health Sciences
Mice
Morphology
Multienzyme Complexes - genetics
Multienzyme Complexes - metabolism
Neonates
Newborn infants
People and Places
Physiological aspects
Population
Population number
Progesterone Reductase - genetics
Progesterone Reductase - metabolism
Reproductive health
Research and analysis methods
Retention
Stereology
Steroid Isomerases - genetics
Steroid Isomerases - metabolism
Testis
Testosterone
Transcription
Transcription (Genetics)
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Summary:The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically established the size of the Leydig cell population, to try to identify transcriptional biomarkers indicative of population size. Following characterisation of the Leydig cell population after Sertoli cell ablation in neonatal life or adulthood, we identified Hsd3b1 transcript levels as a potential indicator of Leydig cell number with utility for informing decision-making on whether to engage in time-consuming stereological cell counting analysis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0219524