Chloroplast and mitochondrial genetic variation of larches at the Siberian tundra-taiga ecotone revealed by de novo assembly

• Published in: PloS one 2019-07, Vol.14 (7), p.e0216966-e0216966
• Main Authors: Zimmermann, Heike H, Harms, Lars, Epp, Laura S, Mewes, Nick, Bernhardt, Nadine, Kruse, Stefan, Stoof-Leichsenring, Kathleen R, Pestryakova, Luidmila A, Wieczorek, Mareike, Trense, Daronja, Herzschuh, Ulrike
• Format: Article
• Language: English
• Subjects: Analysis; Bar codes; Biochemistry; Biology; Biology and Life Sciences; Chloroplasts; Chromosome Mapping; Chromosomes; Climate change; Deoxyribonucleic acid; Diagnostic systems; DNA; DNA, Ancient; DNA, Chloroplast - genetics; DNA, Mitochondrial - genetics; DNA, Plant - genetics; Earth Sciences; Ecology and Environmental Sciences; Ecotones; Engineering and Technology; Evolution; Gene sequencing; Genes; Genetic aspects; Genetic diversity; Genetic polymorphisms; Genetic Variation; Genetics, Population; Genome, Chloroplast; Genome, Mitochondrial; Genome, Plant; Genomes; Genomics; Haplotypes; History, Ancient; Larches; Larix - classification; Larix - genetics; Larix sibirica; Lowlands; Mitochondria; Mitochondrial DNA; Novels; Nucleotides; Polymorphism, Single Nucleotide; Population genetics; Population studies; Populations; Research and Analysis Methods; Rivers; Sediments; Siberia; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Taiga; Taiga & tundra; Tundra; Vegetation
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0216966

Larix populations at the tundra-taiga ecotone in northern Siberia are highly under-represented in population genetic studies, possibly due to the remoteness of these regions that can only be accessed at extraordinary expense. The genetic signatures of populations in these boundary regions are therefore largely unknown. We aim to generate organelle reference genomes for the detection of single nucleotide polymorphisms (SNPs) that can be used for paleogenetic studies. We present 19 complete chloroplast genomes and mitochondrial genomic sequences of larches from the southern lowlands of the Taymyr Peninsula (northernmost range of Larix gmelinii (Rupr.) Kuzen.), the lower Omoloy River, and the lower Kolyma River (both in the range of Larix cajanderi Mayr). The genomic data reveal 84 chloroplast SNPs and 213 putatively mitochondrial SNPs. Parsimony-based chloroplast haplotype networks show no spatial structure of individuals from different geographic origins, while the mitochondrial haplotype network shows at least a slight spatial structure with haplotypes from the Omoloy and Kolyma populations being more closely related to each other than to most of the haplotypes from the Taymyr populations. Whole genome alignments with publicly available complete chloroplast genomes of different Larix species show that among official plant barcodes only the rcbL gene contains sufficient polymorphisms, but has to be sequenced completely to distinguish the different provenances. We provide 8 novel mitochondrial SNPs that are putatively diagnostic for the separation of L. gmelinii and L. cajanderi, while 4 chloroplast SNPs have the potential to distinguish the L. gmelinii/L. cajanderi group from other Larix species. Our organelle references can be used for a targeted primer and probe design allowing the generation of short amplicons. This is particularly important with regard to future investigations of, for example, the biogeographic history of Larix by screening ancient sedimentary DNA of Larix.