Physiological changes of growth hormone during lactation in pup rats artificially reared

• Published in: PloS one 2019-08, Vol.14 (8), p.e0220853-e0220853
• Main Authors: Toriz, Cesar G, Melo, Angel I, Solano-Agama, Carmen, Gómez-Domínguez, Edgar Giovanhi, Martínez-Muñoz, Ma de Los Angeles, Castañeda-Obeso, Jorge, Vera-Aguilar, Eunice, Aguirre-Benítez, Elsa Liliana, Romero-Aguilar, Lucero, González-Del Pliego, Margarita, Jiménez-Estrada, Ismael, Luna, Maricela, Pardo, Juan Pablo, Camacho, Javier, Mendoza-Garrido, Maria Eugenia
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn - blood; Animals, Newborn - growth & development; Animals, Newborn - physiology; Artificial nutrition; Biology and Life Sciences; Blood Glucose - analysis; Breastfeeding & lactation; Carbohydrates; Deprivation; Dopamine; Energy metabolism; Feeding; Female; Food; Food and nutrition; Gene expression; Ghrelin; Ghrelin - blood; Glucose; Growth hormone; Growth Hormone - blood; Growth hormones; Hormones; Insulin; Insulin-like growth factor I; Insulin-Like Growth Factor I - analysis; Insulin-like growth factors; Laboratory rats; Lactation; Lactation - physiology; Liver; Liver - chemistry; Male; Maternal behavior; Maternal Deprivation; Medicine and Health Sciences; Messenger RNA; Metabolism; Phenols (Class of compounds); Physical Sciences; Physiological aspects; Pituitary Gland - cytology; Pituitary Gland - metabolism; Prenatal development; Rats; Rats, Wistar - blood; Rats, Wistar - growth & development; Rats, Wistar - physiology; Real-Time Polymerase Chain Reaction; Research and Analysis Methods; RNA; Secretion; Serum levels; Social behavior; Somatotropin; Tibia - growth & development; Weaning
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0220853

During the lactation period, rat pups are fed by the dam, and the patterns of mother-pup interaction change during this period. Additionally, there are changes in feeding; first, mother´s milk is the only food needed for sustenance, and later, it is combined with solid food and water. GH serum concentrations depend on both maternal-pup interaction and energy metabolism. In the artificial rearing (AR) procedure, pups are deprived of mother-pup interaction, and the feeding pattern is controlled. This rearing paradigm has been used in rats to analyze the effects of maternal deprivation on social behavior. In the present study, we analyzed the variation in GH, acylated ghrelin and IGF-1 serum concentrations throughout the lactation period in AR pups. At pnd7, the maternal rearing (MR) pups responded to a 4 h fast with a drop in GH serum concentration, which is a well-known response to maternal deprivation. GH serum levels in the AR pups did not change, suggesting an adaptation phenomenon. A dopamine inhibitory effect of GH secretion was observed in pnd7 cultured somatotropes, suggesting dopamine regulation of GH secretion at this age. Acylated ghrelin serum levels in the AR pups showed an inverted pattern compared to that in the MR pups, which was related to the artificial feeding pattern. IGF-1 serum levels were lower in the AR pups than in MR pups, which was associated with hepatic GH resistance and with low Igf1 mRNA expression at pnd7. Interestingly, at pnd14, both pup groups showed high hepatic Igf1 mRNA expression but low IGF-1 serum levels, and this was inverted at pnd21. However, serum glucose levels were lower in the AR pups at pnd14 but reached the same levels as the MR pups at pnd21. Moreover, hepatomegaly and higher hepatic GH-receptor levels were observed in the AR pups at pnd21, which was in agreement with an absence of a solid food meal. During AR, the pups lost the maternal interaction-stimulated GH secretion, which correlated with lower IGF-1 serum levels during the first week of postnatal development. Later, the AR pups exhibited hepatic responses, in order to satisfy the metabolic demand for the normal weaning, with low carbohydrates levels in their meal.