Microbiological contamination of young children's hands in rural Bangladesh: Associations with child age and observed hand cleanliness as proxy

• Published in: PloS one 2019-09, Vol.14 (9), p.e0222355-e0222355
• Main Authors: Parvez, Sarker Masud, Azad, Rashidul, Pickering, Amy J, Kwong, Laura H, Arnold, Benjamin F, Rahman, Musarrat Jabeen, Rahman, Md Zahidur, Alam, Mahfuja, Sen, Debashis, Islam, Sharmin, Rahman, Mahbubur, Colford, Jr, John M, Luby, Stephen P, Unicomb, Leanne, Ercumen, Ayse
• Format: Article
• Language: English
• Subjects: Age; Age Factors; Agricultural laborers; Analysis; Biology and Life Sciences; Child health; Children; Children & youth; Cleanliness; Contamination; Courtyards; Dirt; Disease transmission; E coli; Epidemiology; Escherichia coli; Escherichia coli - isolation & purification; Fecal coliforms; Feces; Feces - microbiology; Finger; Fingernail; Food contamination; Food contamination & poisoning; Hand; Hand (anatomy); Hand - microbiology; Hand Disinfection; Hands; Handwashing; Health aspects; Households; Humans; Hygiene; Infant; Medicine; Medicine and Health Sciences; Microbial contamination; Microbiology; Most probable number; Palm; Pathogenic microorganisms; People and Places; Prevention; Proxy; Public health; Risk factors; Rural areas; Sanitation; Sensitivity; Systematic review; Workers
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0222355

Hands are a route of transmission for fecal-oral pathogens. This analysis aimed to assess associations between hand E. coli contamination and child age and determine if observed hand cleanliness can serve as a proxy for E. coli contamination on young children's hands. Trained field workers collected hand rinse samples from children aged 1-14 months in 584 households in rural Bangladesh and assessed the visual cleanliness of child hands (fingernails, finger pads and palms). Samples were analyzed using the IDEXX most probable number (MPN) methodto enumerate E. coli. We assessed if child age (immobile children aged 1-4 months vs. mobile children aged 5-14 months) is associated with log10 E. coli counts on hands using generalized estimating equations (GEE). We estimated the log10 difference in hand E. coli counts associated with the cleanliness of different hand parts using a multivariable GEE model.We calculated the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for dirty fingernails, fingerpads, palms and overall hands (the three observed parts combined) against binary E. coli presence on hands. E. coli was detected on 43% of child hands. Children in the mobile age range had 0.17 log10 MPN higher E. coli on hands than those in the immobile age range (Δlog10 = 0.17, 95% CI = 0.02, 0.32, p = 0.03). Children with visible dirt particles on finger pads had 0.46 log10 MPN higher E. coli on hands than those with clean finger pads (Δlog10 = 0.46, 95% CI = 0.05, 0.87, p = 0.03). Dirty fingernails indicated binary E. coli presence with 81% sensitivity and 26% specificity while dirty fingerpads and palms indicated E. coli presence with 29% sensitivity and 75-77% specificity. The PPV was 45-48% and NPV 59-65% for all three types of observations. Hand contamination with E. coli was prevalent among young children in rural Bangladesh, with higher levels of contamination among mobile children. Studies should assess if strategies to remove animal feces from the courtyard, provide designated hygienic play spaces for children and deliver targeted messaging to mothers to wipe or wash children's hands after contact with animals and animal feces reduce child hand contamination. Visible hand cleanliness was a poor predictor of E. coli presence on young children's hands so other low-cost field measurements are needed to accurately detect fecal contamination on hands.