Exopolysaccharide producing rhizobacteria and their impact on growth and drought tolerance of wheat grown under rainfed conditions

• Published in: PloS one 2019-09, Vol.14 (9), p.e0222302-e0222302
• Main Authors: Khan, Naeem, Bano, Asghari
• Format: Article
• Language: English
• Subjects: Acetic acid; Acids; Ammonia; Ammonia - metabolism; Antioxidants; Antioxidants (Nutrients); Apoptosis; Auxins; Biology and Life Sciences; Burkholderiales - metabolism; Chlorophyll; Chlorophyll - metabolism; Chlorophyll content; Climate change; Crop growth; Crops; Crops (Plants); Cyanides; Disease susceptibility; Drought; Drought resistance; Droughts; Ecology and Environmental Sciences; Environmental aspects; Enzymatic activity; Enzyme activity; Enzymes; Exopolysaccharides; Experiments; Fluorescence; Genotypes; Global warming; Growth hormone; Hormones; Hydrogen; Hydrogen cyanide; Hydrogen Cyanide - metabolism; Indoleacetic acid; Indoleacetic Acids - metabolism; Leaves; Lipid peroxidation; Lipids; Medicine and Health Sciences; Menopause; Metabolites; Moisture; Moisture stress; Nitriles; Organic acids; Performance indices; Peroxidation; Phenols (Class of compounds); Physical Sciences; Physiology; Phytohormones; Plant Development - drug effects; Plant growth; Plant Growth Regulators - metabolism; Plant Leaves - growth & development; Plant Roots - growth & development; Planting; Polysaccharides, Bacterial - metabolism; Polysaccharides, Bacterial - physiology; Population growth; Production management; Productivity; Proline; Proline - metabolism; Rain; Salicylic acid; Salicylic Acid - metabolism; Salinity; Seedlings; Seedlings - growth & development; Seeds; Sensitivity; Soil Microbiology; Somatotropin; Strains (organisms); Stress, Physiological - drug effects; Sugar; Supply and demand; Triticum - growth & development; Triticum - metabolism; Wheat
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0222302

The demand for agricultural crops continues to escalate with an increasing population. To meet this demand, marginal land can be used as a sustainable source for increased plant productivity. However, moisture stress not only affects crop growth and productivity but also induces plants' susceptibility to various diseases. The positive role of plant growth hormone, salicylic acid (SA), on the defence systems of plants has been well documented. With this in mind, a combination of plant growth promoting rhizobacteria (PGPR) and SA was used to evaluate its performance on wheat grown under rainfed conditions (average moisture 10-14%). The selected bacterial strains were characterized for proline production, indole-3-acetic acid (IAA), hydrogen cyanide (HCN), ammonia (NH3), and exopolysaccharides (EPS). Wheat seeds of two genotypes, Inqilab-91 (drought tolerant) and Shahkar-2013 (drought sensitive), which differed in terms of their sensitivity to drought stress, were soaked for three hours prior to sowing in 24-hour old cultures of the bacterial strains Planomicrobium chinense strain P1 (accession no. MF616408) and Bacillus cereus strain P2 (accession no. MF616406). SA was applied (150 mg/L), as a foliar spray on one-month-old wheat seedlings. A significant reduction in the physiological parameters was noted in the plants grown in rainfed conditions but the PGPR and SA treatment effectively ameliorated the adverse effects of moisture stress. The wheat plants treated with PGPR and SA showed significant increases in leaf protein and sugar contents and maintained higher chlorophyll content, chlorophyll fluorescence (fv/fm) and performance index (PI) under rainfed conditions. Leaf proline content, lipid peroxidation, and antioxidant enzyme activity were higher in the non-inoculated plants grown in rainfed conditions but significantly reduced in the inoculated plants of both genotypes. Integrative use of a combination of PGPR strains and SA appears to be a promising and eco-friendly strategy for reducing moisture stress in plants.