Combining fish and environmental PCR for diagnostics of diseased laboratory zebrafish in recirculating systems

Precise knowledge of the health status of experimental fish is crucial to obtain high scientific and ethical standards in biomedical research. In addition to the use of sentinel fish, the examination of diseased fish is a fundamental part of all health monitoring concepts. PCR assays offer excellent...

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Published in:PloS one 2019-09, Vol.14 (9), p.e0222360
Main Authors: Miller, Manuel, Sabrautzki, Sibylle, Beyerlein, Andreas, Brielmeier, Markus
Format: Article
Language:English
Subjects:
Animals
Bacteria - pathogenicity
Bacterial Infections - diagnosis
Bacterial Infections - veterinary
Behavior
Biology and Life Sciences
Danio rerio
Dermatomycoses - diagnosis
Dermatomycoses - veterinary
Detritus
Environmental health
Ethical standards
Fish diseases
Fish Diseases - diagnosis
Fish Diseases - microbiology
Fish Diseases - parasitology
Health
Health monitors
Infections
Laboratories
Laboratory animals
Medical research
Medicine and Health Sciences
Methods
Microsporidiosis - parasitology
Mycobacterium - pathogenicity
Mycobacterium Infections - diagnosis
Mycobacterium Infections - veterinary
Pathogenic microorganisms
Pathogens
Physiology
Polymerase chain reaction
Quarantine
Real-Time Polymerase Chain Reaction - methods
Recirculating systems
Research and Analysis Methods
Sludge
Technology
Tumors
Wastewater-Based Epidemiological Monitoring
Water
Water - analysis
Water purification
Water tanks
Zebrafish
Zebrafish - microbiology
Zebrafish - parasitology
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description Precise knowledge of the health status of experimental fish is crucial to obtain high scientific and ethical standards in biomedical research. In addition to the use of sentinel fish, the examination of diseased fish is a fundamental part of all health monitoring concepts. PCR assays offer excellent sensitivity and the ability to test a broad variety of pathogenic agents in different sample types. Recently, it was shown that analysis of environmental samples such as water, sludge or detritus from static tanks can complement PCR analysis of fish and is actually more reliable for certain pathogens. In our study, we investigated whether the analysis of filtered water mixed with detritus of tanks including fish showing clinical signs of illness is suitable to complement health monitoring programs in recirculating systems. The obtained data indicate that pathogens such as Pseudoloma neurophilia or Myxidium streisingeri were exclusively or mainly found in fish, while mycobacteria were predominantly present in environmental samples. A combination of both sample types seems to be required for the detection of a broad range of infectious agents in zebrafish colonies using real-time PCR technology.
doi_str_mv 10.1371/journal.pone.0222360
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subjects Animals
Bacteria - pathogenicity
Bacterial Infections - diagnosis
Bacterial Infections - veterinary
Behavior
Biology and Life Sciences
Danio rerio
Dermatomycoses - diagnosis
Dermatomycoses - veterinary
Detritus
Environmental health
Ethical standards
Fish diseases
Fish Diseases - diagnosis
Fish Diseases - microbiology
Fish Diseases - parasitology
Health
Health monitors
Infections
Laboratories
Laboratory animals
Medical research
Medicine and Health Sciences
Methods
Microsporidiosis - parasitology
Mycobacterium - pathogenicity
Mycobacterium Infections - diagnosis
Mycobacterium Infections - veterinary
Pathogenic microorganisms
Pathogens
Physiology
Polymerase chain reaction
Quarantine
Real-Time Polymerase Chain Reaction - methods
Recirculating systems
Research and Analysis Methods
Sludge
Technology
Tumors
Wastewater-Based Epidemiological Monitoring
Water
Water - analysis
Water purification
Water tanks
Zebrafish
Zebrafish - microbiology
Zebrafish - parasitology
title Combining fish and environmental PCR for diagnostics of diseased laboratory zebrafish in recirculating systems
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