De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress

Around the world, pansies are one of the most popular garden flowers, but they are generally sensitive to high temperatures, and this limits the practicality of planting them during the warmest days of the year. However, a few pansy germplasms with improved heat tolerance have been discovered or bre...

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Published in:PloS one 2019-09, Vol.14 (9), p.e0222344-e0222344
Main Authors: Du, Xiaohua, Zhu, Xiaopei, Yang, Yaping, Wang, Yanli, Arens, Paul, Liu, Huichao
Format: Article
Language:English
Subjects:
Abiotic stress
Antioxidants
Architectural engineering
Biology and Life Sciences
Biosynthesis
Cultivars
Engineering and Technology
Engineering research
Enzymes
Flowers
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Plant - physiology
Genes
Genes, Plant - genetics
Genes, Plant - physiology
Genetic engineering
Genomes
Germplasm
Heat resistance
Heat shock
Heat shock proteins
Heat stress
Heat tolerance
Heat treatments
Heat-Shock Response
High temperature
High temperatures
Horticulture
Hot Temperature - adverse effects
Inbreeding
Kinases
Landscape architecture
Metabolic pathways
Nucleotides
Oxidative stress
Phenotype
Physical Sciences
Physiology
Real-Time Polymerase Chain Reaction
Regulators
Research and Analysis Methods
Seedlings - metabolism
Seedlings - physiology
Signal transduction
Thermal resistance
Transcription factors
Transcriptome - genetics
Viola - genetics
Viola - metabolism
Viola - physiology
Xiaohua Du
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Wang, Yanli
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Liu, Huichao
description Around the world, pansies are one of the most popular garden flowers, but they are generally sensitive to high temperatures, and this limits the practicality of planting them during the warmest days of the year. However, a few pansy germplasms with improved heat tolerance have been discovered or bred, but the mechanisms of their heat resistance are not understood. In this study, we investigated the transcript profiles of a heat-tolerant pansy inbred line, DFM16, in response to high temperatures using RNAseq. Approximately 55.48 Gb of nucleotide data were obtained and assembled into 167,576 unigenes with an average length of 959 bp, of which, 5,708 genes were found to be differentially expressed after heat treatments. Real-time qPCR was performed to validate the expression profiles of the selected genes. Nine metabolic pathways were found to be significantly enriched, in the analysis of the differentially expressed genes. Several potentially interesting genes that encoded putative transcription regulators or key components involving heat shock protein (HSP), heat shock transcription factors (HSF), and antioxidants biosynthesis, were identified. These genes were highlighted to indicate their significance in response to heat stress and will be used as candidate genes to improve pansy heat-tolerance in the future.
doi_str_mv 10.1371/journal.pone.0222344
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subjects Abiotic stress
Antioxidants
Architectural engineering
Biology and Life Sciences
Biosynthesis
Cultivars
Engineering and Technology
Engineering research
Enzymes
Flowers
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Plant - physiology
Genes
Genes, Plant - genetics
Genes, Plant - physiology
Genetic engineering
Genomes
Germplasm
Heat resistance
Heat shock
Heat shock proteins
Heat stress
Heat tolerance
Heat treatments
Heat-Shock Response
High temperature
High temperatures
Horticulture
Hot Temperature - adverse effects
Inbreeding
Kinases
Landscape architecture
Metabolic pathways
Nucleotides
Oxidative stress
Phenotype
Physical Sciences
Physiology
Real-Time Polymerase Chain Reaction
Regulators
Research and Analysis Methods
Seedlings - metabolism
Seedlings - physiology
Signal transduction
Thermal resistance
Transcription factors
Transcriptome - genetics
Viola - genetics
Viola - metabolism
Viola - physiology
Xiaohua Du
title De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress
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