Differential role of r-met-hu G-CSF on male reproductive function and development in prepubertal domestic mammals

The understanding of mammalian spermatogenesis niche factors active during sexual development may be leveraged to impact reproduction in farm animals. The aim of this study was to evaluate the effects of r-met-hu/G-CSF (filgrastim) on prepubertal sexual development of guinea pigs (Cavia porcellus) a...

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Bibliographic Details
Published in:PloS one 2019-09, Vol.14 (9), p.e0222871-e0222871
Main Authors: Aponte, Pedro M, Gutierrez-Reinoso, Miguel A, Sanchez-Cepeda, Edison G, Garcia-Herreros, Manuel
Format: Article
Language:English
Subjects:
Animal models
Animal welfare
Animals
Animals, Domestic - physiology
Biology and Life Sciences
Blood
Blood cells
Blood-brain barrier
Blood-Brain Barrier - metabolism
Brain research
Cell self-renewal
Cerebrospinal fluid
Cortisol
Dose-Response Relationship, Drug
Filgrastim - administration & dosage
Filgrastim - pharmacokinetics
Gonadotropin-releasing hormone
Gonadotropin-Releasing Hormone - metabolism
Gonadotropins
Granulocyte colony-stimulating factor
Granulocytes
Guinea Pigs
Hematology
Higher education
Laboratory animals
Luteinizing hormone
Male
Mammals
Medicine and Health Sciences
Meiosis
Morphometry
Neurons - drug effects
Neurons - metabolism
Physiology
Pituitary (anterior)
Puberty
Reaction kinetics
Reproduction (biology)
Research and Analysis Methods
Sexual Maturation - drug effects
Sheep, Domestic
Sperm
Spermatogenesis
Spermatogenesis - drug effects
Spermiogenesis
Stem cell transplantation
Stem cells
Steroidogenesis
Stimulation
Testes
Testis - drug effects
Testis - growth & development
Testis - metabolism
Testosterone
Testosterone - metabolism
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Summary:The understanding of mammalian spermatogenesis niche factors active during sexual development may be leveraged to impact reproduction in farm animals. The aim of this study was to evaluate the effects of r-met-hu/G-CSF (filgrastim) on prepubertal sexual development of guinea pigs (Cavia porcellus) and ram lambs (Ovis aries). Individuals of both species were administered r-met-hu/G-CSF daily for 4 days. During and after administration protocols, testicular function and development were assessed through hematological responses, hormonal profiles (gonadotropins, testosterone and cortisol) testicular morphometry and germ cell kinetics. As expected, r-met-hu/G-CSF acutely mobilized white-lineage blood cells in both species. LH was increased by r-met-hu/G-CSF in guinea pigs (P0.05). However, ram lambs showed a dose-response effect regarding testis weight (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0222871