HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer

The primary purpose of this study is to investigate the effect of hedgehog-interacting protein (HHIP) overexpression on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC). Firstly, HHIP gene expression data of NSCLC tissues and normal tissues were obtained from GSE18842/...

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Bibliographic Details
Published in:PloS one 2019-11, Vol.14 (11), p.e0225755-e0225755
Main Authors: Zhao, Jian-Guo, Wang, Jian-Fang, Feng, Jiang-Feng, Jin, Xue-Ying, Ye, Wan-Li
Format: Article
Language:English
Subjects:
Analysis
Biochemistry
Biology and Life Sciences
Biotechnology
Cancer therapies
Carcinoma, Non-Small-Cell Lung - metabolism
Carcinoma, Non-Small-Cell Lung - pathology
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell growth
Cell Line, Tumor
Cell migration
Cell Movement
Cell Proliferation
Chi square analysis
Chi-square test
Cholecystokinin
Data bases
Databases, Factual
Down-Regulation
Drug resistance
Drug stores
Female
Gastric cancer
Gene expression
Genes
Hedgehog-interacting protein
Humans
Lung cancer
Lung diseases
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Male
Medical research
Medicine
Medicine and Health Sciences
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Messenger RNA
Metastasis
Middle Aged
Neoplasm Staging
Non-small cell lung cancer
Non-small cell lung carcinoma
Oncology
Physiology
Polymerase chain reaction
Proteins
RNA
Small cell lung cancer
Small cell lung carcinoma
Statistical tests
Stomach cancer
Tumors
Wounding
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Summary:The primary purpose of this study is to investigate the effect of hedgehog-interacting protein (HHIP) overexpression on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC). Firstly, HHIP gene expression data of NSCLC tissues and normal tissues were obtained from GSE18842/GSE19804/GSE43458 databases of the Gene Expression Omnibus (GEO) database and then validated by TCGA NSCLC database in a cohort of 1027 cases of NSCLC patients and 108 cases of normal people. A chi-square test was used to analyze the relationship between HHIP expression and clinicopathological characteristics of NSCLC. The expression levels of HHIP in NSCLC cells were detected by quantitative-real time PCR. The function of HHIP was investigated by a series of in vitro assays. CCK-8, wounding healing, Transwell invasion assay were utilized to explore the mechanisms of HHIP. HHIP mRNA were significantly down-regulated in NSCLC in three GEO databases and TCGA database (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0225755