Micro-RNA signatures in monozygotic twins discordant for congenital heart defects

MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally. Recent studies demonstrated that miRNAs are involved in the development of congenital heart defects (CHD). In this study, we aimed at identifying the specific patterns of miRNAs in blood of monozygotic twin pairs di...

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Bibliographic Details
Published in:PloS one 2019-12, Vol.14 (12), p.e0226164-e0226164
Main Authors: Abu-Halima, Masood, Weidinger, Josephin, Poryo, Martin, Henn, Dominic, Keller, Andreas, Meese, Eckart, Abdul-Khaliq, Hashim
Format: Article
Language:English
Subjects:
Abundance
Adolescent
Bioinformatics
Biology and life sciences
Blood
Cardiology
Cardiomyocytes
Case-Control Studies
Child
Defects
Deoxyribonucleic acid
Deregulation
Diseases in Twins - genetics
DNA
DNA microarrays
Female
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation
Heart attacks
Heart Defects, Congenital - genetics
Heart failure
Humans
Medicine and Health Sciences
MicroRNAs
MicroRNAs - genetics
miRNA
Oligonucleotide Array Sequence Analysis
Pediatrics
Peripheral blood
Phosphatidylinositol
Post-transcription
Research and Analysis Methods
Ribonucleic acid
RNA
Sequence Analysis, RNA
Signaling
Twins
Twins, Monozygotic - genetics
Young Adult
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Summary:MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally. Recent studies demonstrated that miRNAs are involved in the development of congenital heart defects (CHD). In this study, we aimed at identifying the specific patterns of miRNAs in blood of monozygotic twin pairs discordant for CHD and to assess whether miRNAs might be involved in the development or reflect the consequences of CHD. miRNA microarray analysis and Real-Time Quantitative PCR (RT-qPCR) were employed to determine the miRNA abundance level from 12 monozygotic twins discordant for CHD and their non-CHD co-twins (n = 12). Enrichment analyses of altered miRNAs were performed using bioinformatics tools. Compared with non-CHD co-twins, profiling analysis indicated 34 miRNAs with a significant difference in abundance level (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0226164