Influence of Debaryomyces hansenii on bacterial lactase gene diversity in intestinal mucosa of mice with antibiotic-associated diarrhea

• Published in: PloS one 2019-12, Vol.14 (12), p.e0225802-e0225802
• Main Authors: He, Yunshan, Tang, Yuan, Peng, Maijiao, Xie, Guozhen, Li, Wenge, Tan, Zhoujin
• Format: Article
• Language: English
• Subjects: Alcohol; Aminoglycosides; Animals; Anti-Bacterial Agents - adverse effects; Antibiotics; Bacteria; Bacterial genetics; Base Sequence; Biodiversity; Biology and Life Sciences; Cephradine; Citrobacter; Debaryomyces hansenii; Deoxyribonucleic acid; Diarrhea; Diarrhea - chemically induced; Diarrhea - microbiology; Distilled water; DNA; Drug therapy; Ecology; Ecology and Environmental Sciences; Enzymes; Female; Genes; Genes, Bacterial; Genetic research; Genetic Variation; Gentamicin; Homogeneity; Intestinal Mucosa - microbiology; Intestine; Laboratory animals; Lactase; Lactase - genetics; Lactose; Lysobacter; Male; Medicine; Medicine and Health Sciences; Metabolism; Mice; Microbiota; Mucosa; Phylogeny; Physiology; Principal Component Analysis; Probiotics; Research and analysis methods; Saccharomycetales - physiology; Sulfate; Sulfates; Water
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0225802

The current study aimed to investigate the effects of Debaryomyces hansenii on the diversity of bacterial lactase gene in the intestinal mucosa of antibiotic-associated diarrhea (AAD) mice. Eighteen mice were randomly divided into three groups (6 mice per group): healthy control group, diarrhea model group and D. hansenii treatment group. The antibiotic-associated diarrhea model was established by intragastric administration with a mixture of cephradine and gentamicin sulfate (23.33 mL·kg-1·d-1) twice a day for 5 days continuously. After establishing the AAD model, the mice in the D. hansenii treatment group were gavaged with D. hansenii for three days, while other groups were gavaged with distilled water. Then, the intestinal mucosa of all three groups was collected and DNA was extracted in an aseptic environment for the following analysis. The difference in the richness and homogeneity of the bacterial lactase gene among all samples were inapparent, as the difference in the Chao1, ACE, Simpson and Shannon indices among the three groups were insignificant (P>0.05). NMDS analysis also showed that the distance of the samples among the three groups was unobvious. Furthermore, the bacterial lactase gene in the mucosa mainly originated from Actinobacteria, Firmicutes and Proteobacteria. Compared with the healthy control group, the abundance of lactase genes originating from Cupriavidus, Lysobacter, Citrobacter, Enterobacter and Pseudomonas was increased in the D. hansenii treatment group, while the lactase gene from Acidovorax and Stenotrophomonas decreased (p < 0.01 or p < 0.05) in the diarrhea model group and the D. hansenii treatment group. D. hansenii was capable of improving the growth of some key lactase-producing bacteria like Deinococcus, Cupriavidus and Lysobacter for treating AAD.