The potential role of acrolein in plant ferroptosis-like cell death

• Published in: PloS one 2019-12, Vol.14 (12), p.e0227278-e0227278
• Main Authors: Hajdinák, Péter, Czobor, Ádám, Szarka, András
• Format: Article
• Language: English
• Subjects: Acids; Acrolein; Acrolein - antagonists & inhibitors; Acrolein - metabolism; Apoptosis; Arabidopsis - cytology; Arabidopsis - drug effects; Arabidopsis - physiology; Autophagy; Biochemistry; Biology and Life Sciences; Biotechnology; Carbolines - pharmacology; Carbonyl compounds; Carbonyls; Carnosine; Carnosine - pharmacology; Caspase; Caspases - metabolism; Cell Culture Techniques; Cell death; Cells, Cultured; Cyclohexylamines - pharmacology; Deferoxamine; Deferoxamine - pharmacology; Depletion; Ethanol; Ferroptosis; Ferroptosis - drug effects; Ferroptosis - physiology; Food science; Glutathione; Glutathione - metabolism; Heat; Heat treatment; Iron; Iron - metabolism; Laboratories; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipid Peroxidation - physiology; Lipids; Molecular biology; Mortality; Necrosis; Oxygen; Peroxidation; Peroxide; Phagocytosis; Phenylenediamines - pharmacology; Physical Sciences; Plant cells; Plant Proteins - metabolism; Reactive oxygen species; Research and Analysis Methods; Sodium; Tocopherol; Tumors; Vitamin E
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0227278

The iron dependent, programmed cell death, ferroptosis was described first in tumour cells. It showed distinct features from the already known cell death forms such as apoptosis, necrosis and autophagy. The caspase independent cell death could be induced by the depletion of glutathione by erastin or by the inhibition of the lipid peroxide scavenger enzyme GPX4 by RSL3 and it was accompanied by the generation of lipid reactive oxygen species. Recently, ferroptosis-like cell death associated to glutathione depletion, lipid peroxidation and iron dependency could also be induced in plant cells by heat treatment. Unfortunately, the mediators and elements of the ferroptotic pathway have not been described yet. Our present results on Arabidopsis thaliana cell cultures suggest that acrolein, a lipid peroxide-derived reactive carbonyl species, is involved in plant ferroptosis-like cell death. The acrolein induced cell death could be mitigated by the known ferroptosis inhibitors such as Ferrostatin-1, Deferoxamine, α-Tocopherol, and glutathione. At the same time acrolein can be a mediator of ferroptosis-like cell death in plant cells since the known ferroptosis inducer RSL3 induced cell death could be mitigated by the acrolein scavenger carnosine. Finally, on the contrary to the caspase independent ferroptosis in human cells, we found that caspase-like activity can be involved in plant ferroptosis-like cell death.