Preliminary evidences of the presence of extracellular DNA single stranded forms in soil

The relevance of extracellular DNA (eDNA) in the soil ecosystem is becoming more and more evident to the scientific community by the progressive discovery of functions accompanying to natural gene transformation. However, despite the increased number of published articles dedicated to eDNA in soil,...

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Bibliographic Details
Published in:PloS one 2020-01, Vol.15 (1), p.e0227296-e0227296
Main Authors: Pathan, Shamina Imran, Arfaioli, Paola, Ceccherini, Maria Teresa, Ascher-Jenull, Judith, Pietramellara, Giacomo
Format: Article
Language:English
Subjects:
Adsorption
Bias
Biology and Life Sciences
Computer and Information Sciences
Deoxyribonucleic acid
Discrimination
DNA
DNA, Circular - isolation & purification
DNA, Single-Stranded - isolation & purification
Ecology and Environmental Sciences
Ecosystems
EDTA
Endangered & extinct species
Environmental DNA
Environmental DNA - isolation & purification
Food
Forestry
Genes
Italy
Methods
Physical Sciences
Research and analysis methods
Soil - chemistry
Soil investigations
Soils
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Summary:The relevance of extracellular DNA (eDNA) in the soil ecosystem is becoming more and more evident to the scientific community by the progressive discovery of functions accompanying to natural gene transformation. However, despite the increased number of published articles dedicated to eDNA in soil, so far only few are focused on its single stranded form (eDNAss). The present paper is the first to investigate the quantitative relevance of eDNAss in the total soil eDNA pool, discriminating between its linear (eDNAssl) and circular (eDNAssc) forms and the respective weakly (wa) and tightly (ta) adsorbed fractions. The results showed the prevalence of eDNAss and its linear form in both the total soil eDNA pool and its wa and ta fractions. Both of the eDNAss fractions (linear and circular) were characterized by small fragments.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0227296