Molecular prevalence of Bartonella, Babesia, and hemotropic Mycoplasma species in dogs with hemangiosarcoma from across the United States

Hemangiosarcoma (HSA), a locally invasive and highly metastatic endothelial cell neoplasm, accounts for two-thirds of all cardiac and splenic neoplasms in dogs. Bartonella spp. infection has been reported in association with neoplastic and non-neoplastic vasoproliferative lesions in animals and huma...

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Bibliographic Details
Published in:PloS one 2020-01, Vol.15 (1), p.e0227234
Main Authors: Lashnits, Erin, Neupane, Pradeep, Bradley, Julie M, Richardson, Toni, Thomas, Rachael, Linder, Keith E, Breen, Matthew, Maggi, Ricardo G, Breitschwerdt, Edward B
Format: Article
Language:English
Subjects:
Amplification
Angiogenesis
Animals
Antibodies
Babesia
Babesia - genetics
Babesiosis - epidemiology
Babesiosis - parasitology
Bacteria
Bacterial infections
Bartonella
Bartonella - genetics
Bartonella Infections - epidemiology
Bartonella Infections - microbiology
Bartonella Infections - veterinary
Biology and Life Sciences
Biopsy
Blood
Cancer
Consortia
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Dog Diseases - epidemiology
Dog Diseases - microbiology
Dog Diseases - parasitology
Dogs
Endothelial cells
Female
Genomics
Heart
Hemangiosarcoma - epidemiology
Hemangiosarcoma - microbiology
Hemangiosarcoma - parasitology
Hemangiosarcoma - veterinary
Infections
Inflammation
Laboratories
Male
Medical prognosis
Medicine and Health Sciences
Metastases
Metastasis
Mycoplasma
Mycoplasma - genetics
Mycoplasma Infections - epidemiology
Mycoplasma Infections - veterinary
Neoplasia
Neoplasms
Nucleotide sequence
Oncology
Pathogenesis
Pathogens
Polymerase Chain Reaction
Prevalence
Research and Analysis Methods
Retrospective Studies
Spleen
Surgery
Tissues
Tumors
United States - epidemiology
Vascular endothelial growth factor
Veterinarians
Veterinary colleges
Veterinary medicine
Viruses
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Summary:Hemangiosarcoma (HSA), a locally invasive and highly metastatic endothelial cell neoplasm, accounts for two-thirds of all cardiac and splenic neoplasms in dogs. Bartonella spp. infection has been reported in association with neoplastic and non-neoplastic vasoproliferative lesions in animals and humans. The objective of this study was to determine the prevalence of Bartonella spp. in conjunction with two other hemotropic pathogens, Babesia spp. and hemotropic Mycoplasma spp., in tissues and blood samples from 110 dogs with histopathologically diagnosed HSA from throughout the United States. This was a retrospective, observational study using clinical specimens from 110 dogs with HSA banked by the biospecimen repository of the Canine Comparative Oncology and Genomics Consortium. Samples provided for this study from each dog included: fresh frozen HSA tumor tissue (available from n = 100 of the 110 dogs), fresh frozen non-tumor tissue (n = 104), and whole blood and serum samples (n = 108 and 107 respectively). Blood and tissues were tested by qPCR for Bartonella, hemotropic Mycoplasma, and Babesia spp. DNA; serum was tested for Bartonella spp. antibodies. Bartonella spp. DNA was amplified and sequenced from 73% of dogs with HSA (80/110). In contrast, hemotropic Mycoplasma spp. DNA was amplified from a significantly smaller proportion (5%, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0227234