Comparison of the myometrial transcriptome from singleton and twin pregnancies by RNA-Seq

Preterm birth is recognized as the primary cause of infant mortality worldwide. Twin pregnancies are significantly more at risk of preterm birth than singleton pregnancies. A greater understanding of why this is and better modes of treatment and prevention are needed. Key to this is determining the...

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Bibliographic Details
Published in:PloS one 2020-01, Vol.15 (1), p.e0227882-e0227882
Main Authors: Arrowsmith, Sarah, Fang, Yongxiang, Sharp, Andrew
Format: Article
Language:English
Subjects:
Adult
Age
Biology and Life Sciences
Biosynthesis
Birth
Breech presentation
Cell differentiation
Cesarean Section
Chemokines
Childbirth & labor
Contraction
Down-regulation
Encyclopedias
Enrichment
Female
Fetuses
Gene expression
Gene Expression Regulation - genetics
Gene regulation
Gene sequencing
Genes
Genomes
Genomics
Gestational age
Humans
Immune response
Immune system
Immunity, Innate - genetics
Infant
Infant mortality
Infant, Newborn
Infants
Inflammation - genetics
Lymphocytes
Lymphocytes T
Medicine and Health Sciences
Myometrium
Myometrium - metabolism
Myometrium - pathology
Pregnancy
Pregnancy, Twin - genetics
Pregnant women
Premature birth
Premature Birth - genetics
Premature Birth - pathology
Premature infants
Proteins
R&D
Research & development
Research and analysis methods
Ribonucleic acid
RNA
RNA sequencing
Sequence Analysis, RNA
Signal transduction
Steroids
Studies
T cells
Transcriptome - genetics
Twins
Uterus
Womens health
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Summary:Preterm birth is recognized as the primary cause of infant mortality worldwide. Twin pregnancies are significantly more at risk of preterm birth than singleton pregnancies. A greater understanding of why this is and better modes of treatment and prevention are needed. Key to this is determining the differing pathophysiological mechanisms of preterm birth in twins, including the role of the myometrium and premature uterine contraction. We performed RNA sequencing (RNA-Seq) of human myometrium from singleton and twin pregnancies at term (> 37+0 weeks) and preterm (< 37+0 weeks), collected during pre-labour Caesarean Section. RNA-Seq libraries were prepared from polyA-selected RNA and sequenced on the Illumina HiSeq 4000 platform. Differentially expressed genes (DEGs), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment were conducted using R software. Significance was determined with a false discovery rate-adjusted P value of
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0227882