Occurrence and multilocus genotyping of Giardia duodenalis in captive non-human primates from 12 zoos in China

• Published in: PloS one 2020-02, Vol.15 (2), p.e0228673-e0228673
• Main Authors: Zhang, Xueping, Wang, Liqin, Lan, Xinting, Dan, Jiaming, Ren, Zhihua, Cao, Suizhong, Shen, Liuhong, Deng, Junliang, Zuo, Zhicai, Yu, Shumin, Wang, Ya, Ma, Xiaoping, Liu, Haifeng, Zhou, Ziyao, Hu, Yanchun, Fu, Hualin, He, Changliang, Geng, Yi, Gu, Xiaobin, Peng, Guangneng, Wang, Yufei, Zhong, Zhijun
• Format: Article
• Language: English
• Subjects: Analysis; Animal diseases; Animals; Animals, Zoo - parasitology; Biology and Life Sciences; China - epidemiology; Deoxyribonucleic acid; DNA; Feces; Feces - parasitology; Genetic diversity; Genotype; Genotypes; Genotyping; Giardia; Giardia duodenalis; Giardia lamblia - genetics; Giardiasis - epidemiology; Giardiasis - veterinary; Glutamate; Glutamate dehydrogenase; Health aspects; Heterogeneity; Infection; Infections; Laboratories; Monkeys & apes; Monosaccharides; Municipalities; Novels; Phosphates; Phylogeny; Potassium; Primates; Protozoa; Protozoan Proteins - genetics; Research and Analysis Methods; Studies; Veterinarians; Veterinary colleges; Veterinary medicine; Wildlife; Zoonoses; Zoos
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0228673

Giardia duodenalis is a common enteric protozoan that infects a range of hosts including humans and other mammals. Multilocus genotyping of G. duodenalis in captive non-human primates (NHPs) from zoos in China is limited. In this study, we evaluated 302 NHP fecal samples collected from 32 different NHP species. The primates were from 12 zoos distributed across eight provinces and two municipalities (Chongqing and Beijing) of China. The overall infection rate was 8.3% (25/302). The six G. duodenalis-positive zoos and their infection rates were: Suzhou Zoo (40.0%, 4/10), Yangzhou Zoo (22.2%, 2/9), Dalian Zoo (16.7%, 4/24), Chengdu Zoo (12.8%, 6/47), Guiyang Forest Wildlife Zoo (12.1%, 7/58), and Changsha Zoo (4.7%, 2/43). Molecular analysis of three loci, beta-giardin (bg), triose phosphate isomerase (tpi), and glutamate dehydrogenase (gdh), showed high genetic heterogeneity, and seven novel subtypes (BIII-1, MB10-1, WB8-1, B14-1, MB9-1, DN7-1, and BIV-1) were detected within assemblage B. Additional analysis revealed 12 different assemblage B multilocus genotypes (MLGs), one known MLG and 11 novel MLGs. Based on phylogenetic analysis, 12 assemblage B MLGs formed two main clades, MLG-SW (10-12, 18) and MLG-SW (13, 14, 16, 17), the other four MLG-SW (15, 19, 20, 21) were scattered throughout the phylogenetic tree in this study. Using multilocus genotyping, this study expands our understanding of the occurrence of Giardia infection and genetic variation in Giardia in captive non-human primates from zoos in China.