Phylogenetic analysis reveals an ancient gene duplication as the origin of the MdtABC efflux pump

The efflux pumps from the Resistance-Nodulation-Division family, RND, are main contributors to intrinsic antibiotic resistance in Gram-negative bacteria. Among this family, the MdtABC pump is unusual by having two inner membrane components. The two components, MdtB and MdtC are homologs, therefore i...

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Published in:PloS one 2020-02, Vol.15 (2), p.e0228877-e0228877
Main Authors: Górecki, Kamil, McEvoy, Megan M
Format: Article
Language:English
Subjects:
Analysis
Antibiotic resistance
Antibiotics
Bacteria
Bacterial Proteins - genetics
Biology and Life Sciences
Computer and Information Sciences
Drug Resistance, Multiple, Bacterial - genetics
E coli
Efflux
Evolution
Gene duplication
Gene Duplication - genetics
Genes
Genetic research
Genomes
Gram-negative bacteria
Homology
Medicine and Health Sciences
Membrane Transport Proteins - genetics
Membranes
Microbial drug resistance
Neighborhoods
Nodulation
Phylogenetics
Phylogeny
Proteins
Proteobacteria - genetics
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pumps
Reproduction (copying)
Research and Analysis Methods
Wildlife conservation
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description The efflux pumps from the Resistance-Nodulation-Division family, RND, are main contributors to intrinsic antibiotic resistance in Gram-negative bacteria. Among this family, the MdtABC pump is unusual by having two inner membrane components. The two components, MdtB and MdtC are homologs, therefore it is evident that the two components arose by gene duplication. In this paper, we describe the results obtained from a phylogenetic analysis of the MdtBC pumps in the context of other RNDs. We show that the individual inner membrane components (MdtB and MdtC) are conserved throughout the Proteobacterial species and that their existence is a result of a single gene duplication. We argue that this gene duplication was an ancient event which occurred before the split of Proteobacteria into Alpha-, Beta- and Gamma- classes. Moreover, we find that the MdtABC pumps and the MexMN pump from Pseudomonas aeruginosa share a close common ancestor, suggesting the MexMN pump arose by another gene duplication event of the original Mdt ancestor. Taken together, these results shed light on the evolution of the RND efflux pumps and demonstrate the ancient origin of the Mdt pumps and suggest that the core bacterial efflux pump repertoires have been generally stable throughout the course of evolution.
doi_str_mv 10.1371/journal.pone.0228877
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Among this family, the MdtABC pump is unusual by having two inner membrane components. The two components, MdtB and MdtC are homologs, therefore it is evident that the two components arose by gene duplication. In this paper, we describe the results obtained from a phylogenetic analysis of the MdtBC pumps in the context of other RNDs. We show that the individual inner membrane components (MdtB and MdtC) are conserved throughout the Proteobacterial species and that their existence is a result of a single gene duplication. We argue that this gene duplication was an ancient event which occurred before the split of Proteobacteria into Alpha-, Beta- and Gamma- classes. Moreover, we find that the MdtABC pumps and the MexMN pump from Pseudomonas aeruginosa share a close common ancestor, suggesting the MexMN pump arose by another gene duplication event of the original Mdt ancestor. 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subjects Analysis
Antibiotic resistance
Antibiotics
Bacteria
Bacterial Proteins - genetics
Biology and Life Sciences
Computer and Information Sciences
Drug Resistance, Multiple, Bacterial - genetics
E coli
Efflux
Evolution
Gene duplication
Gene Duplication - genetics
Genes
Genetic research
Genomes
Gram-negative bacteria
Homology
Medicine and Health Sciences
Membrane Transport Proteins - genetics
Membranes
Microbial drug resistance
Neighborhoods
Nodulation
Phylogenetics
Phylogeny
Proteins
Proteobacteria - genetics
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pumps
Reproduction (copying)
Research and Analysis Methods
Wildlife conservation
title Phylogenetic analysis reveals an ancient gene duplication as the origin of the MdtABC efflux pump
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