DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics

To prevent the transmission of damaged genomic material between generations, cells require a system for accommodating DNA repair within their cell cycles. We have previously shown that Escherichia coli cells subject to a single, repairable site-specific DNA double-strand break (DSB) per DNA replicat...

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Bibliographic Details
Published in:PLoS genetics 2020-01, Vol.16 (1), p.e1008473-e1008473
Main Authors: White, Martin A, Darmon, Elise, Lopez-Vernaza, Manuel A, Leach, David R F
Format: Article
Language:English
Subjects:
Biology
Biology and life sciences
Cell cycle
Cell Division
Cell size
Chromosome replication
Chromosomes
Chromosomes, Bacterial - genetics
Deoxyribonucleic acid
DNA
DNA biosynthesis
DNA Breaks, Double-Stranded
DNA damage
DNA repair
DNA replication
Double-strand break repair
E coli
Escherichia coli
Genomes
Genomics
Growth rate
Homeostasis
Nucleoids
Population growth
Recombinational DNA Repair
Replication initiation
Research and Analysis Methods
Septum
Size distribution
Time
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Summary:To prevent the transmission of damaged genomic material between generations, cells require a system for accommodating DNA repair within their cell cycles. We have previously shown that Escherichia coli cells subject to a single, repairable site-specific DNA double-strand break (DSB) per DNA replication cycle reach a new average cell length, with a negligible effect on population growth rate. We show here that this new cell size distribution is caused by a DSB repair-dependent delay in completion of cell division. This delay occurs despite unperturbed cell size regulated initiation of both chromosomal DNA replication and cell division. Furthermore, despite DSB repair altering the profile of DNA replication across the genome, the time required to complete chromosomal duplication is invariant. The delay in completion of cell division is accompanied by a DSB repair-dependent delay in individualization of sister nucleoids. We suggest that DSB repair events create inter-sister connections that persist until those chromosomes are separated by a closing septum.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1008473