Evaluation of systemic inflammatory response and lung injury induced by Crotalus durissus cascavella venom

• Published in: PloS one 2020-02, Vol.15 (2), p.e0224584-e0224584
• Main Authors: Azevedo, Elen, Figueiredo, Ricardo Gassmann, Pinto, Roberto Vieira, Ramos, Tarsila de Carvalho Freitas, Sampaio, Geraldo Pedral, Bulhosa Santos, Rebeca Pereira, Guerreiro, Marcos Lázaro da Silva, Biondi, Ilka, Trindade, Soraya Castro
• Format: Article
• Language: English
• Subjects: Alveoli; Animals; Atelectasis; Biological response modifiers; Biology; Biology and Life Sciences; Bites (Injuries); Crotalid Venoms - pharmacology; Crotalus; Crotalus durissus; Cytokines; Cytokines - analysis; Edema; Experiments; Helper cells; Hemorrhage; Immune response; Immunology; Immunomodulation; Inflammation; Inflammatory response; Inoculation; Interferon; Interferon gamma; Interleukin 10; Interleukin 17; Interleukin 4; Interleukin 6; Interleukins; Laboratories; Lesions; Leukocytes; Leukocytes (polymorphonuclear); Lung diseases; Lung Injury - chemically induced; Lungs; Lymphocytes; Lymphocytes T; Medicine and Health Sciences; Metastases; Mice; Necrosis; Peritoneal fluid; Peritoneum; Physical Sciences; Pulmonary lesions; Rattlesnakes; Systemic Inflammatory Response Syndrome - chemically induced; T-Lymphocytes, Helper-Inducer - metabolism; Time; Time Factors; Tumor necrosis factor; Tumors; Venom; Venoms; Windows (intervals); γ-Interferon
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0224584

This study investigated the systemic inflammatory response and mechanism of pulmonary lesions induced by Crotalus durissus cascavella venom in murine in the state of Bahia. In order to investigate T helper Th1, Th2 and Th17 lymphocyte profiles, we measured interleukin (IL) -2, IL-4, IL-6, IL-10, IL-17, tumor necrosis factor (TNF) and interferon gamma (IFN-γ) levels in the peritoneal fluid and macerated lungs of mice and histopathological alterations at the specific time windows of 1h, 3h, 6h, 12h, 24h and 48h after inoculation with Crotalus durissus cascavella venom. The data demonstrated an increase of acute-phase cytokines (IL-6 and TNF) in the first hours after inoculation, with a subsequent increase in IL-10 and IL-4, suggesting immune response modulation for the Th2 profile. The histopathological analysis showed significant morphological alterations, compatible with acute pulmonary lesions, with polymorphonuclear leukocyte (PMN) infiltration, intra-alveolar edema, congestion, hemorrhage and atelectasis. These findings advance our understanding of the dynamics of envenomation and contribute to improve clinical management and antiophidic therapy for individuals exposed to venom.