NAA and 6-BA promote accumulation of oleanolic acid by JA regulation in Achyranthes bidentata Bl

• Published in: PloS one 2020-02, Vol.15 (2), p.e0229490-e0229490
• Main Authors: Liu, Yanqing, Tang, Li, Wang, Can, Li, Jinting
• Format: Article
• Language: English
• Subjects: Accumulation; Achyranthes - drug effects; Achyranthes - growth & development; Achyranthes - metabolism; Achyranthes bidentata; Agricultural chemicals; Alkaloids; Amino acids; Analysis; Antifungal agents; Artemisinin; Auxins; Benzyl Compounds - pharmacology; Benzyladenine; Biochemistry; Biology and Life Sciences; Biosynthesis; Biosynthetic Pathways - drug effects; Biosynthetic Pathways - genetics; Carotenoids; Cellular signal transduction; Computer and Information Sciences; Cyclopentanes - metabolism; DNA binding proteins; Gene expression; Gene Expression Regulation, Plant - drug effects; Genes; Genes, Plant; Growth regulators; Herbal medicine; Hormones; Jasmonic acid; Life sciences; Medicinal plants; Medicine, Chinese Traditional; Metabolism; Metabolites; Naphthaleneacetic acid; Naphthaleneacetic Acids - pharmacology; Oleanolic acid; Oleanolic Acid - biosynthesis; Oxylipins - metabolism; Phylogeny; Physical Sciences; Physiological aspects; Plant growth; Plant Growth Regulators - metabolism; Plant hormones; Plants, Medicinal - drug effects; Plants, Medicinal - genetics; Plants, Medicinal - metabolism; Proteins; Purines - pharmacology; Ribonucleic acid; RNA; RNA-Seq; Saponins; Saponins - metabolism; Seeds; Signal transduction; Transcription (Genetics); Transcription factors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0229490

Application of plant growth regulators has become one of the most important means of improving yield and quality of medicinal plants. To understand the molecular basis of phytohormone-regulated oleanolic acid metabolism, RNA-seq was used to analyze global gene expression in Achyranthes bidentata treated with 2.0 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L 6-benzyladenine (6-BA). Compared with untreated controls, the expression levels of 20,896 genes were significantly altered with phytohormone treatment. We found that 13071 (62.5%) unigenes were up-regulated, and a lot of differentially expressed genes involved in hormone or terpenoid biosynthesis, or transcription factors were significantly up-regulated. These results suggest that oleanolic acid biosynthesis induced by NAA and 6-BA occurs due to the expression of key genes involved in jasmonic acid signal transduction. This study is the first to analyze the production and hormonal regulation of medicinal A. bidentata metabolites at the molecular level. The results herein contribute to a better understanding of the regulation of oleanane-type triterpenoid saponins accumulation and define strategies to improve the yield of these useful metabolites.