Analysis of serum inflammatory mediators in type 2 diabetic patients and their influence on renal function

• Published in: PloS one 2020-03, Vol.15 (3), p.e0229765-e0229765
• Main Authors: Araújo, Liliane Silvano, da Silva, Marcos Vinícius, da Silva, Crislaine Aparecida, Borges, Maria de Fátima, Palhares, Heloísa Marcelina da Cunha, Rocha, Laura Penna, Corrêa, Rosana Rosa Miranda, Rodrigues Júnior, Virmondes, Dos Reis, Marlene Antônia, Machado, Juliana Reis
• Format: Article
• Language: English
• Subjects: Adiponectin; Age; Biology and Life Sciences; Care and treatment; Chemokines; Comparative analysis; Correlation; Cytokines; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetics; Discipline; Endocrinology; Enzyme-linked immunosorbent assay; Epidermal growth factor receptors; Health sciences; Hyperglycemia; Immunoassay; Immunology; Inflammation; Interleukin 4; Interleukin 8; Kidney diseases; Laboratories; Leptin; Medicine and Health Sciences; Metabolism; Outpatient care facilities; Parasitology; Pathology; Renal function; Tumor necrosis factor receptors; Tumor necrosis factor-TNF; Type 2 diabetes
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0229765

To evaluate the serum concentrations of inflammatory mediators in patients with type 2 diabetes mellitus (T2DM) with or without renal alteration (RA) function. Serum samples from 76 patients with T2DM and 24 healthy individuals were selected. Patients with T2DM were divided into two groups according to eGFR (> or < 60mL/min/1.73m2). Cytokines, chemokines and adipokines levels were evaluated using the Multiplex immunoassay and ELISA. TNFR1 and leptin were higher in the T2DM group with RA than in the T2DM group without RA and control group. All patients with T2DM showed increased resistin, IL-8, and MIP-1α compared to the control group. Adiponectin were higher and IL-4 decreased in the T2DM group with RA compared to the control group. eGFR positively correlated with IL-4 and negatively with TNFR1, TNFR2, and leptin in patients with T2DM. In the T2DM group with RA, eGFR was negatively correlated with TNFR1 and resistin. TNFR1 was positively correlated with resistin and leptin, as well as resistin with IL-8 and leptin. Increased levels of TNFR1, adipokines, chemokines and decrease of IL-4 play important role in the inflammatory process developed in T2DM and decreased renal function. We also suggest that TNFR1 is a strong predictor of renal dysfunction in patients with T2DM.