Diversity in sea buckthorn (Hippophae rhamnoides L.) accessions with different origins based on morphological characteristics, oil traits, and microsatellite markers

• Published in: PloS one 2020-03, Vol.15 (3), p.e0230356-e0230356
• Main Authors: Li, He, Ruan, Chengjiang, Ding, Jian, Li, Jingbin, Wang, Li, Tian, Xingjun
• Format: Article
• Language: English
• Subjects: Agronomy; Analysis; Berries; Biodiversity; Biology and Life Sciences; Biotechnology; Correlation analysis; Cultivars; Economic importance; Fatty acid composition; Fatty acids; Fruits; Genetic analysis; Genetic diversity; Genetic markers; Germplasm; Heat; Hippophae rhamnoides; Hybrids; Laboratories; Life sciences; Lipids; Loci; Markers; Microsatellites; Morphology; Oils & fats; Origins; Physical characteristics; Polyunsaturated fatty acids; Principal components analysis; Pulp; Seeds; Species diversity; Water conservation
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0230356

Sea buckthorn (Hippophae rhamnoides) is an ecologically and economically important species. Here, we assessed the diversity of 78 accessions cultivated in northern China using 8 agronomic characteristics, oil traits (including oil content and fatty acid composition) in seeds and fruit pulp, and SSR markers at 23 loci. The 78 accessions included 52 from ssp. mongolica, 6 from ssp. sinensis, and 20 hybrids. To assess the phenotypic diversity of these accessions, 8 agronomic fruit traits were recorded and analyzed using principal component analysis (PCA). The first two PCs accounted for approximately 78% of the variation among accessions. The oil contents were higher in pulp (3.46-38.56%) than in seeds (3.88-8.82%), especially in ssp. mongolica accessions. The polyunsaturated fatty acid (PUFA) ratio was slightly lower in the seed oil of hybrids (76.06%) than that of in ssp. mongolica (77.66%) and higher than that of in ssp. sinensis (72.22%). The monounsaturated fatty acid (MUFA) ratio in the pulp oil of ssp. sinensis (57.00%) was highest, and that in ssp. mongolica (51.00%) was equal to the ratio in the hybrids (51.20%). Using canonical correspondence analysis (CCA), we examined the correlation between agronomic traits and oil characteristics in pulp and seeds. Oil traits in pulp from different origins were correlated with morphological groupings (r = 0.8725, p = 0.0000). To assess the genotypic diversity, 23 SSR markers (including 17 loci previously reported) were used among the 78 accessions with 59 polymorphic amplified fragments obtained and an average PIC value of 0.2845. All accessions were classified into two groups based on the UPGMA method. The accessions of ssp. sinensis and ssp. mongolica were genetically distant. The hybrid accessions were close to ssp. mongolica accessions. The 8 agronomic traits, oil characteristics in seed and pulp oils, and 23 SSR markers successfully distinguished the 78 accessions. These results will be valuable for cultivar identification and genetic diversity analysis in cultivated sea buckthorn.