Intrinsic anti-Stokes emission in living HeLa cells

Intrinsic fluorescence of biological material, also called auto-fluorescence, is a well-known phenomenon and has in recent years been used for imaging, diagnostics and cell viability studies. Here we show that in addition to commonly observed auto-fluorescence, intrinsic anti-Stokes emission can als...

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Bibliographic Details
Published in:PloS one 2020-03, Vol.15 (3), p.e0230441-e0230441
Main Authors: Kacenauskaite, Laura, Gabrielaitis, Dovydas, Bærentsen, Nicolai, Martinez, Karen L, Vosch, Tom, Laursen, Bo W
Format: Article
Language:English
Subjects:
Biological materials
Biology and Life Sciences
Cell culture
Cell viability
Chemical compounds
Emission
Emissions
Excitation
Fluorescence
Fluorophores
Imaging
Microscopy
Mitochondria
Morphology
Phenols
Physical Sciences
Research and analysis methods
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Summary:Intrinsic fluorescence of biological material, also called auto-fluorescence, is a well-known phenomenon and has in recent years been used for imaging, diagnostics and cell viability studies. Here we show that in addition to commonly observed auto-fluorescence, intrinsic anti-Stokes emission can also be observed under 560 nm or 633 nm excitation. The anti-Stokes emission is shown to be spatially located on/in the mitochondria. The findings presented here show that sensitive imaging experiments e.g. single molecule experiments or two-photon excitation imaging can be compromised if intracellular anti-Stokes emission is not accounted for. On the other hand, we suggest that this anti-Stokes emission could be exploited as an additional modality for mitochondria visualization and cell viability investigation even in systems that are already labeled with commonly used fluorophores that rely on normal Stokes-based detection.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0230441