High-purity isolation of rare single cells from blood using a tiered microchip system

We present a two-tiered microchip system to capture and retrieve rare cells from blood samples with high purity. The first module of the system is a high throughput microfluidic interface that is used to immunomagnetically isolate targeted rare cells from whole blood, and discard > 99.999% of the...

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Bibliographic Details
Published in:PloS one 2020-03, Vol.15 (3), p.e0229949-e0229949
Main Authors: Gur, Onur, Chang, Chun-Li, Jain, Rohil, Zhong, Yuan, Savran, Cagri A
Format: Article
Language:English
Subjects:
Amniocentesis
Analysis
Antigens
Biology and Life Sciences
Blood
Cell Line, Tumor
Cell lines
Cell Separation
Characterization
Containers
Deoxyribonucleic acid
DNA
Engineering and Technology
Fetuses
Humans
Integrated circuits
Isolation
Leukocytes
Leukocytes - cytology
Mechanical engineering
Medicine and Health Sciences
Methods
Microarray Analysis
Microchip Analytical Procedures
Microfluidics
Microfluidics - methods
Modules
Nanotechnology
Neoplasms - blood
Neoplasms - pathology
Physical Sciences
Purification
Purity
Research and Analysis Methods
Scientific equipment industry
Semiconductors
Single-Cell Analysis
Trophoblasts
Trophoblasts - cytology
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Summary:We present a two-tiered microchip system to capture and retrieve rare cells from blood samples with high purity. The first module of the system is a high throughput microfluidic interface that is used to immunomagnetically isolate targeted rare cells from whole blood, and discard > 99.999% of the unwanted leukocytes. The second module is a microwell array that furthers the purification by magnetically guiding each cell into a separate well concurrently, and allows individual retrieval of each cell. We demonstrate the design of the system as well as its characterization by experiments using model cell lines that represent circulating fetal trophoblasts. Our results show that single cells can be retrieved with efficiencies and purities as high as 100% within 145 mins.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0229949