Viral DNA genomes in sera of farrowing sows with or without stillbirths

A study was conducted to investigate the serum virome of sows with and without stillbirths after farrowing. Sera from sows with at least one stillbirth or with normal litters were collected immediately after farrowing. Viral DNA was extracted from serum pools and submitted to high throughput sequenc...

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Bibliographic Details
Published in:PloS one 2020-03, Vol.15 (3), p.e0230714-e0230714
Main Authors: Tochetto, Caroline, Muterle Varela, Ana Paula, Alves de Lima, Diane, Loiko, Márcia Regina, Mengue Scheffer, Camila, Pinto Paim, Willian, Cerva, Cristine, Schmidt, Candice, Cibulski, Samuel Paulo, Cano Ortiz, Lucía, Callegari Jacques, Sidia Maria, Franco, Ana Cláudia, Quoos Mayer, Fabiana, Roehe, Paulo Michel
Format: Article
Language:English
Subjects:
Bioinformatics
Biology and life sciences
Deoxyribonucleic acid
DNA
DNA sequencing
DNA viruses
Genomes
Genomics
Medicine and Health Sciences
Microbiomes
Next-generation sequencing
Novels
Pathogens
Research and Analysis Methods
Stillbirth
Viruses
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Summary:A study was conducted to investigate the serum virome of sows with and without stillbirths after farrowing. Sera from sows with at least one stillbirth or with normal litters were collected immediately after farrowing. Viral DNA was extracted from serum pools and submitted to high throughput sequencing. No differences in the proportion of virus-related reads were found in both groups (p > 0.05). A variety of viral DNA genomes were identified, mostly representative of three viral families: Anelloviridae, Circoviridae and Smacoviridae. Besides, a number of novel unclassified circular Rep-encoding single stranded DNA (CRESS DNA) viruses were also identified. These findings suggest that the presence of such viral genomes in sows' sera bears no correlation with stillbirths' occurrence; it seems likely that these constitute part of the normal serum microbiome of sows at farrowing.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0230714