Genetic relatedness of serial rectal isolates of Acinetobacter baumannii in an adult intensive care unit of a tertiary hospital in Kuwait

Acinetobacter baumannii is an opportunistic pathogen of intensive care unit (ICU) patients. A. baumannii colonizes many parts of the body including the gastrointestinal tract. Endemic and epidemic strains are polyclonal. There is no clarity on the origin of polyclonality of A. baumannii. The objecti...

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Bibliographic Details
Published in:PloS one 2020-04, Vol.15 (4), p.e0230976-e0230976
Main Authors: Al-Hashem, Ghayda, Rotimi, Vincent O, Albert, M John
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Analysis
Antibiotics
Biology and Life Sciences
Clarity
Colonization
Deoxyribonucleic acid
DNA
DNA sequencing
Electrophoresis
Epidemics
Gastrointestinal system
Gastrointestinal tract
Gel electrophoresis
Gene sequencing
Genomes
Genomics
Hospital patients
Identification
Intensive care
Kuwait
Laboratories
Medicine and Health Sciences
Methods
Multilocus sequence typing
Mutation
Nosocomial infections
Opportunist infection
Patients
Pulsed-field gel electrophoresis
Rectum
Research and Analysis Methods
Social Sciences
Subgroups
Tigecycline
United Kingdom
Whole genome sequencing
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Summary:Acinetobacter baumannii is an opportunistic pathogen of intensive care unit (ICU) patients. A. baumannii colonizes many parts of the body including the gastrointestinal tract. Endemic and epidemic strains are polyclonal. There is no clarity on the origin of polyclonality of A. baumannii. The objective of the study was to define the genetic relatedness of serial isolates and the origin of polyclonality. Serial rectal isolates from ICU patients whose rectum was colonized on ≥5 sampling occasions were selected. From a total of 32 eligible colonized patients, isolates from a subgroup of 13 patients (a total of 108 isolates) showing different patterns of colonization as revealed by pulsed-field gel electrophoresis (PFGE) were studied. The isolates were analyzed by PFGE pulsotypes, sequence types (STs) by multi-locus sequence typing (MLST) and clonal complex (CC) by eBURST analysis. Serial isolates constituted a mixture of identical, related and unrelated pulsotypes. Analysis by STs and CCs were less discriminatory. The data suggest a combination of an initial colonizing isolate undergoing mutation as well as colonization by independent isolates. Further clarity on the origin of diversity should be better obtained by whole-genome sequencing.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0230976