Small G protein signaling modulator 3 (SGSM3) knockdown attenuates apoptosis and cardiogenic differentiation in rat mesenchymal stem cells exposed to hypoxia

Connexin 43 (Cx43) may be important in cell death and survival due to cell-to-cell communication-independent mechanisms. In our previous study, we found that small G protein signaling modulator 3 (SGSM3), a partner of Cx43, contributes to myocardial infarction (MI) in rat hearts. Based on these prev...

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Bibliographic Details
Published in:PloS one 2020-04, Vol.15 (4), p.e0231272
Main Authors: Jung, Seung Eun, Choi, Jung-Won, Moon, Hanbyeol, Oh, Sena, Lim, Soyeon, Lee, Seahyoung, Kim, Sang Woo, Hwang, Ki-Chul
Format: Article
Language:English
Subjects:
Apoptosis
Biology and Life Sciences
Bone marrow
Cardiomyocytes
Cell death
Cell differentiation
Cell interactions
Cell survival
Comparative analysis
Connexin 43
Coronary artery disease
Differentiation
Diseases
G proteins
Gene expression
Genes
Heart attack
Heart attacks
Heart cells
Heart diseases
Hospitals
Hypoxia
Medical schools
Medicine
Medicine and Health Sciences
Mesenchymal stem cells
Myocardial infarction
Oxygen
Phenotypes
Physical Sciences
Proteins
Regenerative medicine
Signaling
siRNA
Stem cell transplantation
Stem cells
Survival
Time
Transfection
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Summary:Connexin 43 (Cx43) may be important in cell death and survival due to cell-to-cell communication-independent mechanisms. In our previous study, we found that small G protein signaling modulator 3 (SGSM3), a partner of Cx43, contributes to myocardial infarction (MI) in rat hearts. Based on these previous results, we hypothesized that SGSM3 could also play a role in bone marrow-derived rat mesenchymal stem cells (MSCs), which differentiate into cardiomyocytes and/or cells with comparable phenotypes under low oxygen conditions. Cx43 and Cx43-related factor expression profiles were compared between normoxic and hypoxic conditions according to exposure time, and Sgsm3 gene knockdown (KD) using siRNA transfection was performed to validate the interaction between SGSM3 and Cx43 and to determine the roles of SGSM3 in rat MSCs. We identified that SGSM3 interacts with Cx43 in MSCs under different oxygen conditions and that Sgsm3 knockdown inhibits apoptosis and cardiomyocyte differentiation under hypoxic stress. SGSM3/Sgsm3 probably has an effect on MSC survival and thus therapeutic potential in diseased hearts, but SGSM3 may worsen the development of MSC-based therapeutic approaches in regenerative medicine. This study was performed to help us better understand the mechanisms involved in the therapeutic efficacy of MSCs, as well as provide data that could be used pharmacologically.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0231272