Inflammatory-linked changes in CpG island methylation of three opioid peptide genes in a rat model for pain

Expression of the opioid peptide genes proopiomelanocortin (Pomc), proenkephalin (Penk), and prodynorphin (Pdyn), in immune cells plays a key role in endogenous pain control. In a rat model of painful unilateral paw inflammation, we isolated cells from popliteal lymph nodes and evaluated the role of...

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Bibliographic Details
Published in:PloS one 2018-01, Vol.13 (1), p.e0191698-e0191698
Main Authors: Jacobi, Charlotte Louise Justine, Stein, Christoph
Format: Article
Language:English
Subjects:
Biology and life sciences
Cell activation
CpG islands
Deoxyribonucleic acid
DNA
DNA methylation
Endogenous opioids
Franklin, Benjamin (1706-90)
Gene expression
Genes
Immune system
Immunoprecipitation
Inflammation
Lymph nodes
Medicine and Health Sciences
Methylation
Narcotics
Opioids
Pain
Pain management
Peptides
Physical Sciences
Polymerase chain reaction
Prodynorphin
Proenkephalin
Proopiomelanocortin
Transcription (Genetics)
Transcription activation
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Summary:Expression of the opioid peptide genes proopiomelanocortin (Pomc), proenkephalin (Penk), and prodynorphin (Pdyn), in immune cells plays a key role in endogenous pain control. In a rat model of painful unilateral paw inflammation, we isolated cells from popliteal lymph nodes and evaluated the role of CpG island C5-methylation on the transcriptional activation of those genes. Using methylated DNA immunoprecipitation, we sorted gDNA into methylated (me) and non-me fractions and then determined the CpG island methylation status of each fraction via quantitative Real Time-PCR (qRT-PCR). In silico analysis by MethPrimer software identified one CpG island in Pdyn and three each in Pomc and Penk. No substantial changes in C5-methylation of any gene were observed. In conclusion, the CpG island methylation status does not seem to be a key regulator of opioid gene activation in immune cells during peripheral tissue inflammation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0191698