mRNA expression of LRRC55 protein (leucine-rich repeat-containing protein 55) in the adult mouse brain

• Published in: PloS one 2018-01, Vol.13 (1), p.e0191749-e0191749
• Main Authors: Zhang, Ying-Ying, Han, Xue, Liu, Ye, Chen, Jian, Hua, Lei, Ma, Qian, Huang, Yang-Yu-Xin, Tang, Qiong-Yao, Zhang, Zhe
• Format: Article
• Language: English
• Subjects: Anesthesiology; Animals; Biology and Life Sciences; Brain; Brain - metabolism; Calcium; Calcium ions; Cerebellum; Channels; Cytoplasm; Gene expression; Habenula; Hybridization; Hypertension; In Situ Hybridization; Laboratories; Leucine; Localization; Mammals; Medicine and Health Sciences; Membrane Proteins - genetics; Mice; Mice, Inbred C57BL; Nervous system; Physical Sciences; Pons; Potassium; Potassium channels; Potassium channels (calcium-gated); Potassium conductance; Proteins; Research and Analysis Methods; Resistance; RNA, Messenger - genetics
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0191749

LRRC55 (leucine-rich repeat-containing protein 55) protein is an auxiliary γ subunit of BK (Big conductance potassium channel) channels, which leftward shifts GVs of BK channels around 50 mV in the absence of cytosolic Ca2+. LRRC55 protein is also the only γ subunit of BK channels that is expressed in mammalian nervous system. However, the expression pattern of LRRC55 gene in adult mammalian brain remains elusive. In this study, we investigated the distribution of LRRC55 mRNA in the adult mouse brain by using in situ hybridization. We found that LRRC55 mRNA is richly expressed in the adult mouse medial habenula nucleus (MHb), cerebellum and pons. However, the potential role of LRRC55 in MHb and cerebellum could be different based on the function of BK channels in these brain regions.