Bovine dairy complex lipids improve in vitro measures of small intestinal epithelial barrier integrity

Appropriate intestinal barrier maturation is essential for absorbing nutrients and preventing pathogens and toxins from entering the body. Compared to breast-fed infants, formula-fed infants are more susceptible to barrier dysfunction-associated illnesses. In infant formula dairy lipids are usually...

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Bibliographic Details
Published in:PloS one 2018-01, Vol.13 (1), p.e0190839-e0190839
Main Authors: Anderson, Rachel C, MacGibbon, Alastair K H, Haggarty, Neill, Armstrong, Kelly M, Roy, Nicole C
Format: Article
Language:English
Subjects:
Animal fat
Babies
Baby foods
Biology and Life Sciences
Breast
Breastfeeding & lactation
Complex lipids
Electrical resistivity
Epithelium
Food
Grasslands
Health aspects
Infant formulas
Infants
Integrity
Intestine
Intestines
Lactose
Linoleic acid
Lipids
Lipopolysaccharides
Localization
Mannitol
Maturation
Medicine and Health Sciences
Microscopy
Milk
Nutrients
Nutrition
Permeability
Physical Sciences
Physiological aspects
Proteins
R&D
Research & development
Research and Analysis Methods
Toxins
Tumor necrosis factor-α
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Summary:Appropriate intestinal barrier maturation is essential for absorbing nutrients and preventing pathogens and toxins from entering the body. Compared to breast-fed infants, formula-fed infants are more susceptible to barrier dysfunction-associated illnesses. In infant formula dairy lipids are usually replaced with plant lipids. We hypothesised that dairy complex lipids improve in vitro intestinal epithelial barrier integrity. We tested milkfat high in conjugated linoleic acid, beta serum (SureStart™Lipid100), beta serum concentrate (BSC) and a ganglioside-rich fraction (G600). Using Caco-2 cells as a model of the human small intestinal epithelium, we analysed the effects of the ingredients on trans-epithelial electrical resistance (TEER), mannitol flux, and tight junction protein co-localisation. BSC induced a dose-dependent improvement in TEER across unchallenged cell layers, maintained the co-localisation of tight junction proteins in TNFα-challenged cells with increased permeability, and mitigated the TEER-reducing effects of lipopolysaccharide (LPS). G600 also increased TEER across healthy and LPS-challenged cells, but it did not alter the co-location of tight junction proteins in TNFα-challenged cells. SureStart™Lipid100 had similar TEER-increasing effects to BSC when added at twice the concentration (similar lipid concentration). Ultimately, this research aims to contribute to the development of infant formulas supplemented with dairy complex lipids that support infant intestinal barrier maturation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0190839