Drosophila insulin-like peptide 2 mediates dietary regulation of sleep intensity

Sleep is a nearly universal behavior that is regulated by diverse environmental stimuli and physiological states. A defining feature of sleep is a homeostatic rebound following deprivation, where animals compensate for lost sleep by increasing sleep duration and/or sleep depth. The fruit fly, Drosop...

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Bibliographic Details
Published in:PLoS genetics 2020-03, Vol.16 (3), p.e1008270-e1008270
Main Authors: Brown, Elizabeth B, Shah, Kreesha D, Faville, Richard, Kottler, Benjamin, Keene, Alex C
Format: Article
Language:English
Subjects:
Amino acids
Biology and Life Sciences
Caffeine
Drosophila
Environmental effects
Food
Fruit flies (Tephritidae)
Homeostasis
Insects
Insulin
Medicine and Health Sciences
Neural circuitry
Neural networks
Neurons
Peptides
Physical Sciences
Physiological aspects
Physiology
Quality
Research and Analysis Methods
Sleep
Sleep deprivation
Software
Sucrose
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Summary:Sleep is a nearly universal behavior that is regulated by diverse environmental stimuli and physiological states. A defining feature of sleep is a homeostatic rebound following deprivation, where animals compensate for lost sleep by increasing sleep duration and/or sleep depth. The fruit fly, Drosophila melanogaster, exhibits robust recovery sleep following deprivation and represents a powerful model to study neural circuits regulating sleep homeostasis. Numerous neuronal populations have been identified in modulating sleep homeostasis as well as depth, raising the possibility that the duration and quality of recovery sleep is dependent on the environmental or physiological processes that induce sleep deprivation. Here, we find that unlike most pharmacological and environmental manipulations commonly used to restrict sleep, starvation potently induces sleep loss without a subsequent rebound in sleep duration or depth. Both starvation and a sucrose-only diet result in increased sleep depth, suggesting that dietary protein is essential for normal sleep depth and homeostasis. Finally, we find that Drosophila insulin like peptide 2 (Dilp2) is acutely required for starvation-induced changes in sleep depth without regulating the duration of sleep. Flies lacking Dilp2 exhibit a compensatory sleep rebound following starvation-induced sleep deprivation, suggesting Dilp2 promotes resiliency to sleep loss. Together, these findings reveal innate resilience to starvation-induced sleep loss and identify distinct mechanisms that underlie starvation-induced changes in sleep duration and depth.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1008270