Dysregulated activities of proline-specific enzymes in septic shock patients (sepsis-2)

The proline-specific enzymes dipeptidyl peptidase 4 (DPP4), prolylcarboxypeptidase (PRCP), fibroblast activation protein α (FAP) and prolyl oligopeptidase (PREP) are known for their involvement in the immune system and blood pressure regulation. Only very limited information is currently available o...

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Bibliographic Details
Published in:PloS one 2020-04, Vol.15 (4), p.e0231555-e0231555
Main Authors: Vliegen, Gwendolyn, Kehoe, Kaat, Bracke, An, De Hert, Emilie, Verkerk, Robert, Fransen, Erik, Jongers, Bart 's, Peters, Esther, Lambeir, Anne-Marie, Kumar-Singh, Samir, Pickkers, Peter, Jorens, Philippe G, De Meester, Ingrid
Format: Article
Language:English
Subjects:
Biochemistry
Biology and Life Sciences
Biomarkers
Blood pressure
Cytokines
Dipeptidyl-peptidase IV
Enzymatic activity
Enzymes
Ethylenediaminetetraacetic acids
Fibroblast activation protein
Fibroblasts
Gene expression
Growth factors
Histology
Immune system
Intensive care
Laboratories
Lysosomal Pro-X carboxypeptidase
Medical diagnosis
Medicine
Medicine and Health Sciences
Oligopeptidase
Peptidase
Peptides
Plasma
Proline
Prolyl oligopeptidase
Proteins
Sepsis
Septic shock
Surgery
Survival
Survival analysis
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Summary:The proline-specific enzymes dipeptidyl peptidase 4 (DPP4), prolylcarboxypeptidase (PRCP), fibroblast activation protein α (FAP) and prolyl oligopeptidase (PREP) are known for their involvement in the immune system and blood pressure regulation. Only very limited information is currently available on their enzymatic activity and possible involvement in patients with sepsis and septic-shock. The activity of the enzymes was measured in EDTA-plasma of patients admitted to the intensive care unit (ICU): 40 septic shock patients (sepsis-2) and 22 ICU control patients after major intracranial surgery. These data were used to generate receiver operating characteristic (ROC) curves. A survival analysis (at 90 days) and an association study with other parameters was performed. PRCP (day 1) and PREP (all days) enzymatic activities were higher in septic shock patients compared to controls. In contrast, FAP and DPP4 were lower in these patients on all studied time points. Since large differences were found, ROC curves were generated and these yielded area under the curve (AUC) values for PREP, FAP and DPP4 of 0.88 (CI: 0.80-0.96), 0.94 (CI: 0.89-0.99) and 0.86 (CI: 0.77-0.95), respectively. PRCP had a lower predicting value with an AUC of 0.71 (CI: 0.58-0.83). A nominally significant association was observed between survival and the DPP4 enzymatic activity at day 1 (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0231555